This research focuses on the effects of polyphenols and carotenoids found in Green tea and Cloves on inhibiting and treating pathogenic infections. Pathogenic infections are on the rise and cases are being reported from around the world with a mortality rate of 60%-65% [1,2]. It is suspected that the number of people infected with pathogenic infections will rise by 10%-15% in the next two decades
. Most pathogenic infections such as Escherichia coli, Pseudomonas aeruginosa and Staphylococcus haemolyticus are antibiotic resistant towards levofloxacin based medications, which are the most commonly used medications for such infections (refer to Table 1). Generally, antibiotics for these pathogens are penicillin…show more content… Hypothesis
It is hypothesized that Camellia sinensis and Syzygium aromaticum will be very effective in inhibiting the cell growth of these pathogenic infections due to the enrichment of polyphenols.
Inhibiting Pathogenic Infections: A Polyphenol and Carotenoid Based Approach 2 of 7 carotenoids. It is also hypothesised that the crude extracts of the polyphenols and carotenoids will not perform as well because these two compounds have a synergistic effect on each other.
A three-phased experiment was conducted over several months.
Phase 1 - In the first phase the anti-bacterial effects of Camellia sinensis and Syzygium aromaticum were tested on Enterobacter aerogenes. The results showed that Camellia sinensis and Syzygium aromaticum were able to inhibit the cell growth of the pathogen with zero side effects, i.e., antibacterial effects on the bacteria. The experiment was conducted in a school lab. Bacteria growth and bacteria killed were measured through microscopic imaging.
Phase 2 - In the second phase of the experiment, the anti-bacterial effects of Camellia sinensis and
Syzygium aromaticum were tested against Pseudomonas aeruginosa, Escherichia coli and
Staphylococcus haemolyticus. From the observations it was inferred that polyphenols and carotenoids found in Camellia sinensis and Syzygium