The Identification of Green Fluorescence Protein Tagged Kinesin Mutations in Caenorhabditis Elegans Through Fluorescence Microscopy

Muscle contraction can be understood as the consequence of a process of transmission of action potentials from one neuron to another. A chemical called acetylcholine is the neurotransmitter released from the presynaptic neuron. As the postsynaptic cells on the muscle cell membrane receive the acetylcholine, the channels for the cations sodium and potassium are opened. These cations produce a net depolarization of the cell membrane and this electrical signal travels along the muscle fibers. Through the movement of calcium ions, the muscle action potential is taken into actual muscle contraction with the interaction of two types of proteins, actin and myosin.

As well as these there are also the axon of the cell which is covered in myelin sheaths which carried information away from the cell body and hands the action potentials, these are small short bursts of change in the electrical charge of the axon membrane through openings of ion channels, off to the following neurons dendrites through terminal buttons at the end of the axons. Whenever an action potential is passed through these terminal buttons it releases a chemicals that pass on the action potential on to the next neuron through the terminal button and dendrite connection. The chemicals that are

Wischusen, William, Jolissaint, Ann, Reiland, Jane, and Pomarico, Steven. 2012. Biology 1208/1209: Biological Laboratories for Science Majors. Hayden McNeil, Plymouth,

To make the specimen compatible with both forms of advanced microscopy, they sufficiently prepared samples by coupling the specimen with a fluorescence that was also conductive. This technique was accomplished with the FlouroNanogold label, which contains gold nanoparticles covalently bonded to a fluorescence label. That way, the LM worked as well as the EM for the same set of kinetochores that were being studied. The Hec1 protein was stained in this case because this protein naturally delineates the structures to be studied.

The cell body comprises of the nucleus and other organelles (Ward, 2010). The nucleus contains the genetic code, and this is involved with protein synthesis (He, 2013). The dendrites receive information from other neurons which are located in a close proximity (Kalat, 1995). The terminal of an axon compresses into a disc-shaped structure (Gross, 2010). This is where chemical signals also known as a neurotransmitter permit interaction amongst neurons, by means of a minute gap named a synapse (Martin, Carlson & Buskit, 2013). Both neurons which form the synapse are referred to as a presynaptic synapse (prior to the synapse) and postsynaptic (after the synapse), reflecting the direction of information flow (from axon to dendrite), (He, 2013).

A light microscope was set up with the light on a low setting; one large Daphnia was selected and placed in the centre of a cavity slide by using a pipette.

The main components of the synapses are as follows: The Axon terminal, found at the end of the Axon, passes neurotransmitters to other neurons via synaptic transmission. Synaptic Vesicles contain neurotransmitters within the Axon. Neurotransmitters themselves are chemical messengers that travel through the neurons and activate receptors on the receiving cell. The neurotransmitters are diffused through the synaptic cleft—a region between the two neurons and gap the neurotransmitter needs to cross to make it to the receiving cell. Said receiving cell is what receives the neurotransmitters and starts the process over again. The receptors on the cell are structures that receive the neurotransmitters and

The structure of neuromuscular junction consists of a neuron and skeletal muscle cell. The motor neurons, which arise from the spinal cord, supply the skeletal muscle fibers. The neuromuscular junction is un-myelin nerve with a bulb shape at the endings that contract the muscle fiber. The schwann cells form a covering over the postsynaptic membrane and nerve membrane of the fiber that is located under the terminal and is categorized as a post-junction folds. The area between the folds and the bulbs create the synaptic cleft. This consists of proteins and proteoglycans. The enzyme acetylcholinesterase; exist only at high levels in the synaptic basal lamina (UMN,

Molecules move around the cells through vessels. There are three different ways that molecules enter the cell diffusion, facilitated diffusion and active transport. Diffusion is when a molecule moves from high concertation to low concentration. Facilitated diffusion is when the molecules goes through a plasma membrane. Active transport is when an organism uses energy to move molecules. The three scientist discovered the different aspects to make sure and understand the research of making sure that the right cargo is shipped to the correct destination at exactly the right time. The scientist use the example of a pancreas. The pancreatic cells make insulin and release it in the blood. Chemical signals called neurotransmitters are sent from one nerve cell to another, this allow humans to have some of the functions they have today. The discoverers discovered that the molecules move at a fast pace within the cell and also they used the comparison of rush hour traffic to the molecules moving within the cells. Dr. Schekman discovered a set of genes that were required for vesicle traffic. To discover better research on this experiment he compared two cells, one that was normal and one that was mutated. Dr. Rothman discovered protein machinery that allows vesicles to join with their targets to go through with the transfer of cargo. Proteins on the vesicle bind to specific distinguished proteins on the target membrane, to make sure that the vesicle joins at the specific location and that cargo molecules are delivered to the correct destination. Dr. Südhof revealed how signals give orders to vesicles to release their cargo with precision. He studied how signals are directed to one nerve cell to another. He also discovered how calcium controls this process and that it controls certain things at the

As the message arrives at the end of the nerves, the message is transmitted to the muscles. Before the message is transmitted to the muscles it has to pass the space between the end of the nerve and the muscle, and that space is called neuromuscular junction. The message is transmitted from the brain to the end of the nerve and from the nerve to the neuromuscular junction, and when the message arrives the chemical called neurotransmitters are released.

Thoughts that started in the cerebral cortex as synapses between unmylientated neurons of many brilliant gentleman. These synapses traveled to other parts of the brain to turn into synapses that signal movement of certain muscle fibers. Movement that occurs by a signal accompanied by chemical elements traveling down millions of dendrites, myelinated axons, and axon terminals. They reach the muscle fibers where synaptic vessels release acetylcholine into the synaptic clef where the ACH(acetylcholine) travels down the sarcolemma into the T tubules into the SR which releases Calcium ions. The Calcium ions then attach to protein myosin which attaches to tripoponin a protein part of actin. The myosin contracts moving the actin and then more acetylcholine is produced so the myosin unhooks from the actin. These muscle

In order to accomplish all this, the following optical methods were used to determine the nature and the extent of the attack, and the defining anatomical characteristics of the xylophagic species that have attacked the icon: optical microscopy (UV and IR) and SEM.

As soon as the electrical signal reaches the end of the axon, mechanism of chemical alteration initiates. First, calcium ion spurt into the axon terminal, leading to the release of neurotransmitters “molecules released neurons which carries information to the adjacent cell”. Next, inside the axon terminal, neurotransmitter molecules are stored inside a membrane sac called vesicle. Finally, the neurotransmitter molecule is then discharged in synapse space to be delivered to post synaptic neuron.

Nerve cells generate electrical signals to transmit information. Neurons are not necessarily intrinsically great electrical conductors, however, they have evolved specialized mechanisms for propagating signals based on the flow of ions across their membranes.

Look at the photo of a Pacinian corpuscle. Notice the onion-like bulb of connective tissue. Describe briefly —