Tudy, We Have Investigated The Activities Of Seven Terpenic

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tudy, we have investigated the activities of seven terpenic compounds; namely carvacrol, cinnamaldehyde, citral, eugenol, geraniol, menthol and thymol against C. tropicalis biofilm. Interestingly, it was found that citral, geraniol, and thymol were significantly effective against planktonic cells of C. tropicalis when compared to C. albicans. Antifungal activities of citral and geraniol were found to be similar with MIC50 (32ug/ml) while thymol was found to have half the MIC50 value (16ug/ml) against planktonic cells of C. tropicalis than C. albicans. The minimum inhibitory concentration (MIC50) is defined as the concentration with 50% cell growth in contrast to control or untreated sample. When the microdilution format is utilized and…show more content…
albicans and other non-albicans candida species biofilms 32, no expanded study has been conducted on the activity of terpenes against C. tropicalis biofilm so far. Here, we have explored the biofilm inhibition and biofilm eradication properties of citral, geraniol, and thymol against C. tropicalis. Biofilm inhibition concentration (BIC50) and biofilm eradication concentration (BEC50) was defined as the concentration at which 50% of the biofilm cells are metabolically active in contrast to the untreated sample. The calculated BIC50 was found to be two folds higher than MIC50 in presence of citral (64 ug/ml) , geraniol (64 ug/ml) and thymol (32 ug/ml) , whereas, BEC50 (128ug/ml) of matured biofilm is two to four folds effective when treated with citral, geraniol, and thymol. In the case of azole standard drugs such as fluconazole, Ramage et al. [38], has demonstrated that biofilm cells were also resistant with MICs increased 64–1000 times compared with planktonic cells. Even higher concentrations are needed for complete eradication of biofilm as it forms a firm habitat for its survival. Thus, there is a need to look for better compounds which are natural and less harmful when to be used against candida biofilms. Because there is no consensus regarding the acceptable inhibition level for natural products, we can say the undergone study could be considered well with the definition proposed by Morels et al. [34]. If extracts displayed a MIC less
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