Antiseptic Lab

Rubbing alcohol, also known as isopropyl alcohol “refers to two water-soluble chemical compounds—ethyl alcohol and isopropyl alcohol—that have generally underrated germicidal characteristics” (“Guideline for Disinfection and Sterilization in Healthcare Facilities”). Ethyl alcohol kills bacteria in two ways: “protein denaturation and dissolving the lipid membrane” (“How Does Ethyl Alcohol Kill Bacteria?“). Next, Betadine, also known as Povidone-iodine is a solution [that is] often used in hand scrubs and hand rubs in hospital settings” (Ngan). It is used in “Treating minor wounds and infections, as well as killing bacteria” (“Povidone/ iodine solution”). Povidone-iodine’s way of killing bacteria is still being researched and “is yet to be fully understood, [but] it appears to have the ability to penetrate the cell wall of bacteria and affect their structure and function. This ensures their rapid destruction and helps protect against wound and skin infection” (“What is an Antiseptic”). Germ-X Hand Sanitizer is a hand gel that is used for waterless “washing” of hands to kill germs on the surface. It is an antiseptic primarily used by those who want to kill germs quickly and cannot wash their hands (Samuels). The formula contains about 63 percent ethyl alcohol according to livestrong.com (Samuels). Neosporin is a topical antibiotic used to

THE EFFECT OF INCREASING THE CONCENTRATION OF DETTOL ANTISEPTIC LIQUID ON THE GROWTH OF E.COLI

Modern-day advances have allowed scientists to develop methods to control pathogens. The more common methods include the use of antiseptics, antibiotics, and vaccines. By definition, antiseptics are chemicals used to kill pathogens. A few widespread antiseptics include soap, vinegar, and rubbing alcohol. Antiseptics are primarily used for the external destruction of pathogens, while antibiotics and vaccines target internal pathogens. Nevertheless, there are and can be difficulties concerning the use of antibiotics. “As antibiotic use has become more common, antibiotic-resistant bacteria have evolved.” This resistance due to evolution is called antibiotic resistance. It results in the antibiotics having no effect whatsoever on the bacteria, rendering the antibiotics useless. When antibiotic resistance occurs, scientists must resume their search to create a new medicine to can kill the mutant bacteria.

AIM – The aim of the experiment is to determine the relative effectiveness of several anti-microbial substances on developing pathogens. (E. coli)

2. Which antibiotic was most effective in killing the bacterial lawn on each plate? Which was the least effective?

Antibiotics either stop the bacterial cell from reproducing or kill the cell. They can disrupt the bacteria by deterring

The aim of this investigation was to find out which antiseptics were most effective at preventing the growth of bacteria.

5) My hypothesis: “The higher the concentration of antiseptic, the more powerful it will be at inhibiting/ killing microorganisms which cause bacterial growth.” This theory supports my result because as you can see the zone of inhibition increases due to the increasing concentration of antiseptic. The higher concentration of antiseptic means it has been less diluted, which means its more stronger than other more diluted concentrations, this high concentration will determine how powerful the antiseptic will be at killing/inhibiting the growth of bacterium, this high concentration of antiseptic will break down the bacterial cell wall, after this process has happened the antiseptic can stop the mutation of the bacteria and inhibit the

The disinfectant that was the most effective was the Wescodyne® due to the fact that it was the only disinfectant that had a zone of inhibition for both E. coli and S. aureus, and the zone of inhibition was quite large, with it being 16 millimeters for E. coli and it being 19 millimeters for S. aureus.

Chemical antimicrobial agents are chemical compounds capable of either inhibiting the growth of microorganisms or killing them outright. Those which are taken internally to alleviate the symptoms of or promote healing from disease are called chemotherapeutic drugs, and among these is a class of compounds called antibiotics. In order for a chemotherapeutic drug to be classed as an antibiotic, it must be produced by a microorganism such as bacterium or fungus or at least derived from a chemical produced by one. It must also be capable of killing or inhibiting the growth of other microorganisms and of doing so when taken in very small quantities.

Chemical methods of control: Antimicrobial drugs, involves the use of chemicals to prevent and treat infectious diseases. Pasteur and others observed that infecting an animal with Pseudomonas aeruginosa protected the animal against Bacillus anthracis. Later, the word “antibiosis” (against life) for this inhibition and called the inhibiting substance “Antibiotic”. As researchers found out more and more about these chemicals they were able to discover that antibiotics are chemicals or natural substances such as bacteria, fungi, as well as synthetic drugs; that inhibit or kill microbes in small amounts. Chemotherapeutic agents are antimicrobial chemicals (natural or synthetic) that can be used internally or may be absorbed.

After 48 hours, the experimenter was able to obtain the Petri dish in order to observe what had taken place, within the nutrient agar plate, of the span of this period of time. The experimenter was able to establish that any clear areas surrounding the filter-paper disks were the zones of inhibition and they indicated that the antibiotic was able to inhibit the bacterial growth, and any cloudy areas of the agar demonstrated that bacterial growth was still able to take place within the given conditions. Thus, the larger the zone of inhibition, the more bacteria was inhibited within this specific environment. Quadrant 1 contained an Erythromycin filter-paper disk, which was able to inhibit the growth of Bacteria D. The Erythromycin filter-paper

Disease and plague had once completely and disastrously overwhelmed humanity for countless years. During the era when medicine was not as advanced and sickness and disease had run rampant, people had resorted to dangerous, unethical and unsanitary methods of healing. Desperate to alleviate the pain, doctors and healers of the time did what they could to make the pain and discomfort of the ill disappear. Though the intentions of many doctors was to heal the sickly, they did not realize there was a more scientific approach of solving many of their problems. On a microscopic level, there are bacteria and microbes that are attacking the body, causing diseases to ensue. In order to deal with the issue at hand, scientists had to figure out what could be used to fight these particular pathogens and not just what, but how much of these antimicrobials should be used. To better understand this idea of knowing the proper dosage needed for an antibiotic, we exercised this concept of minimal inhibitory concentration. The minimal inhibitory concentration is the lowest amount of the antimicrobial concentration needed to prevent the noticeable growth of the microorganism after incubating the sample overnight. Each group was given a specific antibiotic of

Have you ever experienced putting any sort of treatment on a scrape, cut, or wound? Well Joseph Lister's concept of antiseptics helped influence that concept. Antiseptics helps with the limitation of the spread of bacteria and germs. This concept came from the idea that bacteria and germs were responsible for the infections people developed after surgery. This bacteria could normally come from the air, but also came from uncleaned equipment, unwashed hands, and dirty surgical aprons or clothing. For this conflict Lister developed antiseptics which were medicines that slowed or stopped the growth of bacteria. One of his contributions was an antiseptic chemical called, carbolic acid. This cleaned wounds after surgeries, it was also sprayed in

This lab and its procedures are all about finding out the unknown identification of a given bacteria. The lab consists of specific techniques, tests, chemicals, and vocabulary that are necessary for the finding of the bacterial identity. A bacterium is randomly assigned and it is a group effort to find the bacteria name through many of its specialties and characteristics. An example of classifying it would be to determine whether the bacteria is catalase negative or positive, or if the species is gram negative or positive. This lab is of huge significance because of its medical microbiology connections. Scientists Gurtler and Stanisich explained the connections more eloquently. They stated, in their medical article, that, “Medical microbiology