preview

What Is The Gene Of The CALLAR ) Gene?

Decent Essays

The phylogeny shows the relationship of different CASP genes that code for different Caspase proteins in different eukaryotic organisms. This tree was created using CFLAR as the outgroup; (Dereeper, 2008). CFLAR is naturally the most distantly related gene to the others, however CFLAR is in fact quite similar to CASP8. The proteins they both produce are structurally similar, for instance they both have DED domains; (Stennicke & Salvesen, 2000; Watt et al., 1999). Conversely, the protein CFLAR encodes, lacks Caspase activity and is involved in the process of anti-apoptosis, which contrasts the function of CASP8; (Frank et al., 2006). The “CASP” genes form a monophyletic group, as they are all involved in causing apoptosis, whereas CFLAR …show more content…

This effect may be due to the loss of its hydrophobicity due to the missense mutation, and thus may alter the function of the active site of the enzyme.

Similarly, an SNP of CASP8 with a G->C mutation at position 904, substituting histidine to aspartic acid, has been associated with reducing the risk of breast cancer (MacPherson et al., 2004). Although, both amino acids have similar hydrophilic properties, making this mutation synonymous. Therefore, this requires more research to investigate the intricacies of the protein when the residue is changed.
To amplify CASP8-212 (one of the protein coding regions in the CASP8 gene) by RT-PCR, the gene’s cDNA sequence was obtained from the Ensembl database and then run through a primer-BLAST; (Yates et al., 2016; Ye et al., 2012). Out of the 4 primers listed in Table 1, primer 3 would be recommended after conducting a cost-benefit analysis; one reason being due to the GC% being low at 55% for both forward and reverse primers, being better than primers 2 & 4 that have a GC% greater than 55%. Having a substantial GC%, makes the PCR inefficient, due to excessive costs. While denaturing nucleic acid during PCR, the temperature is raised; and considering that GC are joined via 3 H-bonds, and AT only 2, GC would require higher temperatures to break them. Also primer 3 has a higher product length than primer 5 (which that has the same

Get Access