. To find genes that are turned on or off in response tochanges in osmolarity (the total concentration of solutes in solution), you grow a culture of E. coli in amedium with high osmolarity and another culture in amedium with low osmolarity. You now performRNA-Seq analysis on each culture. It is possible thatosmotic changes may induce a general stress responsethat may be seen with other stresses as well (for example, heat shock). How could you distinguish thegenes that might be involved in a general stress response from those that are specific for the osmolaritychange? (Note: You will need to grow additional bacterial cultures.)

. To find genes that are turned on or off in response tochanges in osmolarity (the total concentration of solutes in solution), you grow a culture of E. coli in amedium with high osmolarity and another culture in amedium with low osmolarity. You now performRNA-Seq analysis on each culture. It is possible thatosmotic changes may induce a general stress responsethat may be seen with other stresses as well (for example, heat shock). How could you distinguish thegenes that might be involved in a general stress response from those that are specific for the osmolaritychange? (Note: You will need to grow additional bacterial cultures.)

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter37: The Endocrine System

Section: Chapter Questions

Problem 1VCQ: Figure 37.5 Heat shock proteins (HSP) are so named because they help refold misfolded proteins. In...

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Figure 37.5 Heat shock proteins (HSP) are so named because they help refold misfolded proteins. In response to increased temperature (a “heat shock"), heat shock proteins are activated by release from the NR/HSP complex. At the same time, transcription of HSP genes is activated. Why do you think the cell responds to a heat shock by increasing the activity of proteins that help refold misfolded proteins?
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