.. Tris(hydroxymethyl)aminomethane abbreviated as Tris or TRIŽMA is frequently used to make buffers in biochemistry laboratories. Tris has a Molecular weight 121.1 g/mol and its solution in water is basic with pH 10.5. Describe in details how to prepare 1L of 0.5 M Tris buffer solution at pH 7.5. . If 2L of 0.05 M Tris-HCI buffer solution pH = 7.5 are required for an experiment on column chromatography. Describe in details how to prepare this buffer solution by diluting the buffer prepared in question # 1. %3D

.. Tris(hydroxymethyl)aminomethane abbreviated as Tris or TRIŽMA is frequently used to make buffers in biochemistry laboratories. Tris has a Molecular weight 121.1 g/mol and its solution in water is basic with pH 10.5. Describe in details how to prepare 1L of 0.5 M Tris buffer solution at pH 7.5. . If 2L of 0.05 M Tris-HCI buffer solution pH = 7.5 are required for an experiment on column chromatography. Describe in details how to prepare this buffer solution by diluting the buffer prepared in question # 1. %3D

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter14: Principles Of Neutralization Titrations

Section: Chapter Questions

Problem 14.32QAP

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