1- Are the following changes in conditions likely to increase or decrease retention time? a. faster flow rate b. longer column c. longer connector between column and detector d. higher analyte concentration e. higher temperature f. larger sample size g. using a less polar column while doing a reverse-phase separation h. using a less polar mobile phase while doing a reverse-phase separation

Principles Of Pharmacology Med Assist
6th Edition
ISBN:9781337512442
Author:RICE
Publisher:RICE
Chapter8: Forms Of Drugs And How They Act
Section: Chapter Questions
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1- Are the following changes in conditions likely to increase or decrease retention time?
a. faster flow rate
b. longer column
c. longer connector between column and detector
d. higher analyte concentration
e. higher temperature
f. larger sample size
g. using a less polar column while doing a reverse-phase separation
h. using a less polar mobile phase while doing a reverse-phase separation
2- In what order will the following substances elute?
• In reverse-phase liquid chromatography:
a. butylamine, 2-butylene, ethylamine, ethylene
b. benzene, chlorophenol, phenol, trichlorophenol
• In gas chromatography with a polar column:
c. butanol, methanol, water
• In gas chromatography with a nonpolar column:
d. butanol, methanol, water
• In ion chromatography:
e. bromide, fluoride, sulfide
Transcribed Image Text:1- Are the following changes in conditions likely to increase or decrease retention time? a. faster flow rate b. longer column c. longer connector between column and detector d. higher analyte concentration e. higher temperature f. larger sample size g. using a less polar column while doing a reverse-phase separation h. using a less polar mobile phase while doing a reverse-phase separation 2- In what order will the following substances elute? • In reverse-phase liquid chromatography: a. butylamine, 2-butylene, ethylamine, ethylene b. benzene, chlorophenol, phenol, trichlorophenol • In gas chromatography with a polar column: c. butanol, methanol, water • In gas chromatography with a nonpolar column: d. butanol, methanol, water • In ion chromatography: e. bromide, fluoride, sulfide
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