1. A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to determine the population of the culture, by transferring 25 ml of the suspension to 225 ml 0.90% NaCl. Three consecutive dilutions were further made by using 1 ml aliquot in 9 ml of 0.90% NaCl. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating method.
1. A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to determine the population of the culture, by transferring 25 ml of the suspension to 225 ml 0.90% NaCl. Three consecutive dilutions were further made by using 1 ml aliquot in 9 ml of 0.90% NaCl. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating method.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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i. Illustrate the dilution series used and label the final dilution of each dilution.
Transcribed Image Text:1. A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was
sampled to determine the population of the culture, by transferring 25 ml of the
suspension to 225 ml 0.90% NaCl. Three consecutive dilutions were further made by
using 1 ml aliquot in 9 ml of 0.90% NaCl. One ml from each dilution was plated in
each of duplicate plates. The following table shows the results of the plating
method.
Sampling
COUNTS
1st dilution
54; 61
TNTC
3rd dilution
0; 0
242; 233
TNTC
2nd dilution
4th dilution
1st
2nd
3; 7
TNCT
0,0
28: 37
3rd
TNTC
TNTC
249;246
* TNTC = Too numerous to count
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