A serial dilution of a bacterial culture yields the following number of colonies. Which plate(s) should be used to determine the original cell density? Plate A Plate B Plate C Plate D Plate E Dilution Factor 10-5 10-6 10-7 10-8 10-9 # of Colonies Too many to count 850 456 80 14 Group of answer choices All of these choices A & B D & E E None of these choices A B & C D B C & D C

A serial dilution of a bacterial culture yields the following number of colonies. Which plate(s) should be used to determine the original cell density? Plate A Plate B Plate C Plate D Plate E Dilution Factor 10-5 10-6 10-7 10-8 10-9 # of Colonies Too many to count 850 456 80 14 Group of answer choices All of these choices A & B D & E E None of these choices A B & C D B C & D C

Aquaculture Science

3rd Edition

ISBN:9781133558347

Author:Parker

Publisher:Parker

Chapter2: Aquatic Plants And Animals

Section: Chapter Questions

Problem 2KA

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A 10-5 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 278 colony forming units grow on the plate. How many bacteria are in a single mL of the original culture? Express your answer to two decimal places using scientific notation. In scientific notation 540 would be written as 5.40*10^2. Since only 0.1 mL is put on the plate, this counts as an extra dilution!!! Any time less than 1 mL is transferred, a dilution is being performed. Any time more than 1 mL is transferred, a concentration is being performed. Include the trailing zero so there are two decimal places Canvas expects a single digit before the decimal point. 5.40*10^2 is how Canvas expects 540 to be formatted in scientific notation 54.00*10^1 would be marked wrong. 0.54*10^3 would be marked wrong. A 10-5 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the…
Using a dilution series is a way to transfer the very small volumes of original culture necessary to get countable colony forming units. If you suspect your culture of bacteria has 59.62*10^9 cells per mL, how many mL of original culture would you want to end up on the plate to produce 68 cfu? Express your answer in scientific notation rounded to two decimal places. Eg. 0.01001 would be 1.00*10^-2 You must include trailing zeros 0.0000040001 would be reported as 4.00*10^-6 There must always be two decimal places, even if they are zeros Canvas expects one digit before the decimal point 0.40*10^-5 would be marked incorrect for 0.0000040001
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