bacterial culture has a concentration of 3.2 x 108 cells /mL. You dilute this culture as follows: 1/50, then 10-3and finally 1/20. If you then plate 0.2 mL of the final dilution, how many CFU would you expect following incubation?
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A bacterial culture has a concentration of 3.2 x 108 cells /mL. You dilute this culture as follows: 1/50, then 10-3and finally 1/20. If you then plate 0.2 mL of the final dilution, how many CFU would you expect following incubation?
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- A bacterial culture has a concentration of 3.2 x 108 cells /mL. You dilute this culture as follows: 1/50, then 10-3 and finally 1/20. If you then plate 0.2 mL of the final dilution, how many CFU would you expect following incubation?To dilute a bacterial culture, 500 μl of a 16 hour culture is mixed with fresh culture media to a final volume of 5 ml. How did you calculate it?. Single line text.You have 2 mL of bacterial culture. How would you make a 10^-2 dilution using the entire culture volume
- You place 1 ml of a bacterial broth culture into bottle A containing 99 ml of sterile water and mix. You take 1 ml from bottle A and transfer it to bottle B, which contains 99 ml of sterile water. What is the dilution of the bacterial culture in bottle B?You are asked to make a 1:1000 dilution of a bacterial culture in a final volume of 5000 mL. What should be your initial volume from the original culture? Show solution.You count 4 squares of a hemacytrometer and ontain 82,65,85 cells in each squy.what is the cell density of this culture?
- a pure bacterial culture was diluted by adding a 0.2 mL aliquot to 0.9mL water. Then 0.1 mL of this dilution was plated out, yielding 82 colonies. Calculate the CFU/mL in the original culture.You are asked to add 2 mL of bacterial culture to 98 mL of water. What is your dilution factor? Show solutionIn this experiment, a culture was serially diluted to the concentrations below. Each plate was plated with 0.25mL of the dilution. Using the most diluted plate, what is the correct concentration of the original culture? A) 2.0 X 10^-3 cells/mL B) 2.0 X 10^5 cells/mL C) 4.0 X 10^5 cells/mL D) 5.0 X 10^4 cells/mL
- You aseptically transfer 1 ml of your optional liquid culture into 99 ml of sterile water. What is the dilution factor. Show workUsing absorbance readings and a standard curve relating absorbance to cell number, you suspect that a bacterial culture contains 7.4 x 109 CFUs/ml. What dilution of this sample would give you a countable plate?How would you produce a 10^-1 dilution if a 3 mL bacterial sample using the entire 3 mL volume? suppose your professor handed you a test tube with 2.0 mL of an E. coli broth culture in it and told you to make a 10^-1 dilution of the entire culture. Explain how you would do this. Show your calculations.