1. Alpha-glucosidase has a molar mass of about 40 kilodaltons. If 0.50 grams of the enzyme is dissolved in 250 mL of buffer, calculate Kcat for the enzyme with and without quercetin Note: Vmax for the reaction without quercetin = 0.6666 Vmax for the reaction with quercetin= 0.3333

1. Alpha-glucosidase has a molar mass of about 40 kilodaltons. If 0.50 grams of the enzyme is dissolved in 250 mL of buffer, calculate Kcat for the enzyme with and without quercetin Note: Vmax for the reaction without quercetin = 0.6666 Vmax for the reaction with quercetin= 0.3333

Introduction to General, Organic and Biochemistry

11th Edition

ISBN:9781285869759

Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Chapter23: Enzymes

Section: Chapter Questions

Problem 23.65P

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Question

1. Alpha-glucosidase
has a molar mass of about 40 kilodaltons. If 0.50 grams of the enzyme
is dissolved in 250 mL of buffer, calculate Kcat for the enzyme with and without
quercetin
Note: Vmax for the reaction without quercetin = 0.6666
Vmax for the reaction with quercetin= 0.3333

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