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Q: Would you calculate the ionic strength of this buffer? [Na2HPO4]=.024176 [NaH2PO4]=.1
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1. calculate the initial pH of the NH3/NH4+ buffer. How does it compare to the experimental value?
2. calculate the pH of the NH3/NH4+ buffer after the addition of HCl. How does it compare to the experimental value.
3. calculate the pH of the NH3/NH4Cl buffer after the addition of NaOH. How does it compare to the experimental value?
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- Make 50 ml of the extraction buffer containing the following ingredients using the stock solution you made (1M Tris-HCl, 5 M NaCl, 0.5 M EDTA, and 10% SDS). You add each solution into one sterile tube using sterile pipets and add an appropriate amount of sterile ddH2O to make it 50 ml. Include the amount of sterile ddH2O. Extraction buffer composition: 200 mM Tris-HCl (pH 8.0) 2 M NaCl 25 mM EDTA (pH 8.0) 1% SDSwhy is it necessary to test the ph? EXTRME DETAIL PLEASE PROCEDURE To a six inch test tube was added thiamine hydrochloride (0.65 g), followed by water (2mL). The mixture was gently shaken until homogeneous, and then ethanol (7.5 mL)was added. Finally, sodium hydroxide (10%, 1.5 mL) was added and the mixture wasgently stirred to ensure complete mixing.Benzaldehyde (3.8 mL) was then added at room temperature in one portion with gentlestirring. The pH of the resulting reaction mixture was determined and adjusted to >10by dropwise addition of 10% sodium hydroxide.Standard solution : 0.201 grams of acetylsalicylic acid was combined with 10 mL of 0.5 M NaOH, then transfered to a 100 mL flask and filled the rest of the way with water Then 0.500 mLs of the standard solution was transfered to a 10.00 mL flask and diluted to the 10 mL mark with 0.02 M of buffered iron chloride. This step was repeated using 0.400, 0.300, 0.200, 0.100 mLs of the standard solution Questions: A.) determine the concentration of the acetylsalicylic acid in the 100.00 mL volumetric flask containing the solution from which the standards were made. B.)determine the concentration of the acetylsalicylic acid in each of the 10.00 mL calibration solutions. I only need the work for 0.400 mLs C.)Make a scatter plot (on the computer again) of absorbance versus concentration for the calibration samples. On the plot, add a linear trendline, and display the equation and the R2 value on the chart. Take your time to label the axes
- BUFFER CAPACITY EXPERIMENT Part 1: Preparation and pH Meter Calibration Prepare 50 mL of 0.5 M acetic acid from a concentrated stock. Prepare 50 mL of 0.5 M sodium hydroxide from a solid. Part 2: Buffer Capacity Transfer 20 mL of 0.5 M acetic acid (Ka is 1.75*10-5), stir at a slow setting and measure the pH reading is stabilized. Add 1.0 mL of 0.5 M NaOH and measure the pH. Continue adding 0.5 M NaOH in 0.5 - 1.0 mL increments until a major spike in pH occurs. After the spike, add another two 0.5 – 1.0 mL increments of 0.5 M NaOH. Create a pH vs. Volume graph to determine the range of the buffer’s capacity. Compare the actual data (nonstandard conditions) with the theoretical data under standard conditions. I NEED HELP WITH CALCULATIONS AN SMALL RESULT INTERPRETATION DESCRIPTION PLEASE TO UNDERSTAND WHAT I DID AND WHY THANKSAccurately weigh out about 6g copper(ii) sulfate crystals into a weighing boat. Use the copper(ii) sulfate crystals to make up 250cm3 of standardized copper (ii) sulfate solution Pipette 25cm3 of this solution into a conical flask Add 1.5g potassium iodide and swirl thoroughly Titrate this solution with standard 0.1 moldm-3 Na2S2O3 in a burette. When the iodine color fades, add 1 cm3 starch indicator. Use your titration data below to calculate the percentage by mass of copper in the copper(ii) sulfate crystals.1. These are balances that have a maximum loading of 10 to 30 g with a precision of 60.01 mgA. semi-microanalyticalB. MicroanalyticalC. MacrobalancesD. Triple Beam Balance2. Which type of curve is normally produced when plotting the change in the concentration of a specie of some function and the amount of reagent added?A. Titration curveB. Sigmoidal CurveC. Phase Transition curveD. Calibration curve3. In the dead dear case study, silver diethyldithiocarbamateforms a colored solution when it binds with arsenic, what do we call substance like silver diethyldithiocarbamate?A. Secondary standard reagentB. Precipitating agentC. Chelating agentD. Primary standard reagent
- You want to make a solution of the primary standard sodium oxalate. It has a molecular mass of 133.999 g/mol (no error). Your analytical balance has an error of 0.2 mg. You add some weighing paper to the balance and it reads a mass of 0.23626 g. You then add sodium oxalate onto the weighing paper until the mass registers as 2.8539 g. You carefully transfer this to a 250.0 ml volumetric flask with an error of 0.1 ml and dissolve the sodium oxalate in a sufficient volume of water. What is the concentration of the oxalic acid (in M) and what is the absolute error (also in M).A deepwell water sample of 100 mL was titrated with 0.010 M EDTA at pH 10 ( with Mg-EDTA pH 10 buffer reagent) and consumed 31 mL. Calculate the Total Hardness of the water sample as mg CaCO3. Ans in 3 sig figures. no need to write the unit.How would you make up 1 L of a 20X stock solution of the following buffer? 50mM glucose (FW=180.16)25mM Tris (FW 121.1)10mM EDTA (FT=372.24) I think, I already calculated the grams of each one, but I don't know how to do the 20X Glucose: 9.008gTris: 3.02gEDTA:3.72G
- create a qulaitative analysis separations flow chart for the separation of Sb3+ and Cd2+Determining the Ksp of Calcium Hydroxide: Ca(OH)2 (s) ↔ Ca2+ (aq) + 2OH– (aq) Ksp = [Ca2+][OH–]2 The molarity of the hydrochloric acid used was 0.050 M.15.00 mL of filtered Ca(OH) 2 was measured out for the titration.Calculate the [Ca 2+ ]. Explain, please. Trial Initial Buret Reading Endpoint Buret Reading Endpoint Volume Equivalence Point Volume 1 80 ml 9.31 pH 12 ml 12.60mlThe water hardness of tap water has been determined to be 120 (= 120 mg CaCO3 per 1 L of water). After the tap water has run through the ion exchange column, titration of a 20.00 mL sample used only 0.70 mL of 0.0100 M EDTA, to reach the endpoint. Calculate the effectiveness of the ion exchange column: what is the % of the calcium, removed by the ion exchange column? Provide your answer in % and without decimal. ( Please type answer note write by hend)