Consider the following set of data and answer the fo 2.Consider the following set of data and answer the following questions:() tslV (umol/min)20.8V (+ Inhibitor) (umol/min)6x 101 x 1052x 1051262.451-6x 1051.8x 10202467.68728a. Plot the data on a Lineweaver-Burk plot (be sure to label axes)b. Determine the Kmc. Defermine the Vmaxd. The second set of velocities represents the rate of the reaction whenan inhibitor is added. Plot these data on the same graph as aboveand determine the new Km and Vmax and the type of inhibitor(competitive, uncompetitive, non-competitive).e. Can the effects of the inhibitor be over-ridden by adding moresubstrate? Why?The kinetics of an enzyme were analyzed in the absence of inhibitors, aswell as in the presence of Inhibitor A and Inhibitor B. Using the given databelow, construct or calculate the following (Make sure to label graphswith appropriate axes and equations, and circle final answers):No InhibitorInhibitor AInhibitor B[S] mMVo (mmol/min) V, (mmol/min)2.401.293.2.403.60128.405.102410.86.3012.07.208.55a. Write out the Michaelis-Menten and Lineweaver-Burke equationsb. Plot all of the data on one plot as Michaelis-Menten şaturation curvesc. Plot all of the data on one plot in the Lineweaver-Burk formatd. Define Km and Vmaxe. Mathematically determine the Km and Vmax in the absence and In thepresence of inhibitorsf. Determine what type of inhibitors A and B are

1. Consider the following set of data and answer the following questions: IS) (M) 6x 10 1x 105 2x 105 6x 105 1.8 x 10 V (umol/min) V (+ Inhibitor) (umol/min) 20.8 12 29 15 45 20 67.6 24 87 28 a. Plot the data on a Lineweaver-Burk plot (be sure to label axes) b. Determine the Km C. Determine the Vmax d. The second set of velocities represents the rate of the reaction when an inhibitor is added. Plot these data on the same graph as above and detemine the new Km and Vmax and the type of inhibitor (competitive, uncompetitive, non-competitive). e. Can the effects of the inhibitor be over-ridden by adding more substrate? Why?

1. Consider the following set of data and answer the following questions: IS) (M) 6x 10 1x 105 2x 105 6x 105 1.8 x 10 V (umol/min) V (+ Inhibitor) (umol/min) 20.8 12 29 15 45 20 67.6 24 87 28 a. Plot the data on a Lineweaver-Burk plot (be sure to label axes) b. Determine the Km C. Determine the Vmax d. The second set of velocities represents the rate of the reaction when an inhibitor is added. Plot these data on the same graph as above and detemine the new Km and Vmax and the type of inhibitor (competitive, uncompetitive, non-competitive). e. Can the effects of the inhibitor be over-ridden by adding more substrate? Why?

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter16: Adult And Pediatric Dosages Based On Body Surface Area

Section: Chapter Questions

Problem 5.4P

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