In a spectrophotometer, what should be the appearance on the graph if the sample is pure? presence of salt and protein?
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In a spectrophotometer, what should be the appearance on the graph if the sample is pure? presence of salt and protein?
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- In the spectrophotometer, why is there a need to measure a 'blank' sample? What will happen to the accuracy of your result if you measure the specimens without measuring a 'blank' trial? In the experiment, what liquid can be placed in the cuvette when measuring a 'blank' trial?What biological molecules can you measure a concentration of using a spectrophotometer?what is 3 ocular units, 4.2 ocular units, 5 ocular units, 3.6 ocular units, and 8 ocular units in micrometers
- Why is it important to select immiscible solvents when performing liquid-liquid extraction? In general, how can you determine which layer (aqueous or organic) is on top, and which is on the bottom?What is the purpose of setting the spectrophotometer to zero absorbance using a blank solution?Isoelectric focusing is used to separate …………….. based on their ................ A. salts, solubility B. nucleic acid, size C. proteins, charge D. proteins, size (molecular mass) E. molecules, density
- If a researcher forgot to wipe the outside a sample cuvette to make it free of drops, fingerprints, and dust prior to reading it in the spectrophotometer, how might the absorbance value of the sample be affected? (Higher, Lower, No Change) Explainhow can you determine aluminum, lead and zinc in a specimen using a microchemical technique?Two compounds have Rf values of 0.50 and 0.61, respectively. The solvent front on a plate is developed to 10 cm. How far apart in centimeters are the spots for the two compounds?
- Why does one see methyl orange as orange in color? What blank would be used to standardize this spectrophotometer?You have a 20 mg/mL stock solution of the amino acid arginine and need to make plates containing arginine at a concentration of 10 micrograms/mL. How much of the stock solution do you add to 1 liter of plate media?What is the reagent used to detect glucose in the ph and temperature experiments? What is its principle? What is the principle of the spectrophotometer?