12:50 0וח.וה...SECTION I1. Epigenetic marks refer to modifications to DNA and proteins that in turn regulate gene expression.All of the following are true regarding epigenetic types of modifications except:A. They have the ability to both silence and activate genes.B. They can involve the methylation of cytosine bases.C. They involved changes to the sequence of DNA in the genomeD. They are linked to the acetylation of histones.E. They can be due to environmental factors2. You are growing a population of cells that appear to be resistant to G418, a chemical that is normallytoxic to cells. Upon further analysis of the cells' genome, you find that it contains a genomicsequence that encodes the protein neomycin phosphotransferase. What would you do to test if thisprotein confers resistance to the G418 chemical?A.Induce expression (i.e., through plasmid cloning) of the gene for neomycinphosphotransferase in a G418-sensitive cell line, then treat these cells with G418Determine if the cells are also making B-galactosidaseSequence the gene using Sanger sequencingLabel a fragment of the gene to see where it is expressedDetermine if an antibody to neomycin phosphotransferase binds to G418B.С.D.E.3. After you remove the transcription activator and run the DNA on a gel, you find that thepromoter sequence is CTGTTC. Which of the four gels represents the one with the promoter?1signal signalsignal signalsignal signalsignal signalA)B)C)D)E) none of themAll of the following are true gxceptA. Nuclear pore complexes are specialized so that some mediate import and others mediate export.B. Membrane-bound and free ribosomes, which are structurally and functionally identical, differonly in the proteins they happen to be making at a particular time.C. Some proteins can be made as either free or membrane-bound proteins. They have the sameMRNA but different splicing and poly-adenylation patterns.D. A protein that does not have a sorting signal will remain in the cytosol.E. A protein cannot leave the ER unless it is correctly folded.5. The outside of a cell contains more solutes than the inside of the cell. Which describes the inside of4.the cell and the direction of water?A. Inside the cell is hypertonic. Water will move towards the outside of the cell.B. Inside the cell is hypertonic. Water will move towards the inside of the cell.C. Inside the cell is isotonic. The net flow of water will be equal in each directionD. Inside the cell is hypotonic. Water will move towards the inside of the cell.E. Inside the cell is hypotonic. Water will move towards the outside of the cell.6. Which of the following statements about prokaryotic cells and eukaryotic cells is true?A. Both cells have replication and transcription, but only eukaryotic cells have translation, splicing,and polyadenylationB. Both cells have replication, transcription, and splicing. but only prokaryotic cells havetranscription and translation occur simultaneouslyC. Both cells have replication, transcription, translation, and splicing, but only eukaryotic cells havepolyadenylation.D. Both cells have replication, transcription, and translation, but only eukaryotic cells have splicingand polyadenylationE Both cells have replication, transcription, and translation, but only eukaryotic cells have Okazakifragments from multiple origins of replication.7. In Sanger Sequencing, which of the following are added to determine where the A's (adenosines) arelocated in the sequence? Note: DNTPS are (DATP, dTTP, dCTP, dGTP) and ddNTPs are (ddATP,ddTTP, ddCTP, ddGTP) 12:49 014. You want to amplify the DNA between two stretches of sequence shown in the figure below. Explainwhich one of the following primer pairs would allow you to amplify the DNA by PCR.DNA to be amplified5'-GACCTGTGGAAGC-CATACGGGATTGA-3"3'-CTGGACACCTTCGGTATGCCCTAACT-5"primers(1) 5'-GACCTGTCCAAGC-3(2) 5-CTGGACACCTTCG-3(5) 5-CATACGGGATTGA-3"(6) 5°-GTATGСССТААСТ-3°(3) 5'-CGAAGGTGTCCAG-3'(7) 5'-TGTTAGGGCATAC-3"(4) 5'-GCTTССАСАGGTC-3°(8) 5'-TCAATCCCGTATG-3'END5THE END

Question

12:50 0וח.וה...SECTION I1. Epigenetic marks refer to modifications to DNA and proteins that in turn regulate gene expression.All of the following are true regarding epigenetic types of modifications except:A. They have the ability to both silence and activate genes.B. They can involve the methylation of cytosine bases.C. They involved changes to the sequence of DNA in the genomeD. They are linked to the acetylation of histones.E. They can be due to environmental factors2. You are growing a population of cells that appear to be resistant to G418, a chemical that is normallytoxic to cells. Upon further analysis of the cells' genome, you find that it contains a genomicsequence that encodes the protein neomycin phosphotransferase. What would you do to test if thisprotein confers resistance to the G418 chemical?A.Induce expression (i.e., through plasmid cloning) of the gene for neomycinphosphotransferase in a G418-sensitive cell line, then treat these cells with G418Determine if the cells are also making B-galactosidaseSequence the gene using Sanger sequencingLabel a fragment of the gene to see where it is expressedDetermine if an antibody to neomycin phosphotransferase binds to G418B.С.D.E.3. After you remove the transcription activator and run the DNA on a gel, you find that thepromoter sequence is CTGTTC. Which of the four gels represents the one with the promoter?1signal signalsignal signalsignal signalsignal signalA)B)C)D)E) none of themAll of the following are true gxceptA. Nuclear pore complexes are specialized so that some mediate import and others mediate export.B. Membrane-bound and free ribosomes, which are structurally and functionally identical, differonly in the proteins they happen to be making at a particular time.C. Some proteins can be made as either free or membrane-bound proteins. They have the sameMRNA but different splicing and poly-adenylation patterns.D. A protein that does not have a sorting signal will remain in the cytosol.E. A protein cannot leave the ER unless it is correctly folded.5. The outside of a cell contains more solutes than the inside of the cell. Which describes the inside of4.the cell and the direction of water?A. Inside the cell is hypertonic. Water will move towards the outside of the cell.B. Inside the cell is hypertonic. Water will move towards the inside of the cell.C. Inside the cell is isotonic. The net flow of water will be equal in each directionD. Inside the cell is hypotonic. Water will move towards the inside of the cell.E. Inside the cell is hypotonic. Water will move towards the outside of the cell.6. Which of the following statements about prokaryotic cells and eukaryotic cells is true?A. Both cells have replication and transcription, but only eukaryotic cells have translation, splicing,and polyadenylationB. Both cells have replication, transcription, and splicing. but only prokaryotic cells havetranscription and translation occur simultaneouslyC. Both cells have replication, transcription, translation, and splicing, but only eukaryotic cells havepolyadenylation.D. Both cells have replication, transcription, and translation, but only eukaryotic cells have splicingand polyadenylationE Both cells have replication, transcription, and translation, but only eukaryotic cells have Okazakifragments from multiple origins of replication.7. In Sanger Sequencing, which of the following are added to determine where the A's (adenosines) arelocated in the sequence? Note: DNTPS are (DATP, dTTP, dCTP, dGTP) and ddNTPs are (ddATP,ddTTP, ddCTP, ddGTP) 12:49 014. You want to amplify the DNA between two stretches of sequence shown in the figure below. Explainwhich one of the following primer pairs would allow you to amplify the DNA by PCR.DNA to be amplified5'-GACCTGTGGAAGC-CATACGGGATTGA-3&#34;3'-CTGGACACCTTCGGTATGCCCTAACT-5&#34;primers(1) 5'-GACCTGTCCAAGC-3(2) 5-CTGGACACCTTCG-3(5) 5-CATACGGGATTGA-3&#34;(6) 5°-GTATGСССТААСТ-3°(3) 5'-CGAAGGTGTCCAG-3'(7) 5'-TGTTAGGGCATAC-3&#34;(4) 5'-GCTTССАСАGGTC-3°(8) 5'-TCAATCCCGTATG-3'END5THE END

Accepted Answer

Different macromolecules are present in humans. They include carbohydrates, lipids, proteins, and…