Biology 2e
2nd Edition
ISBN: 9781947172517
Author: Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher: OpenStax
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Chapter 14, Problem 33CTQ
What is the role of a primer in
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Why do DNA Polymerases Require a Primer?
The following gel was produced in manual DNA sequencing. What would the unknown DNA template be that this gel represents?
During agarose gel electrophoresis, why does DNA move through the gel when electric current is applied?
because DNA is negatively charged
because a charged chemical from the loading buffer is bound to the DNA
because DNA is positively charged
because DNA absorbs electricity
Chapter 14 Solutions
Biology 2e
Ch. 14 - Figure 14.10 In eukaryotic cells, DNA and RNA...Ch. 14 - Figure 14.14 You isolate a cell strain in which...Ch. 14 - Figure 14.21 A fr am eshift mutation that results...Ch. 14 - If DNA of a particular species was analyzed and it...Ch. 14 - The experiments by Hershey and Chase helped...Ch. 14 - Bacterial transformation is a major concern in...Ch. 14 - DNA double helix does not have which of the...Ch. 14 - In eukaryotes, what is the DNA wrapped around?...Ch. 14 - Meselson and Stahl's experiments proved that DNA...Ch. 14 - If the sequence of the 5'-3' strand is AATGCTAC,...
Ch. 14 - How did Meselson and Stahl support Watson and...Ch. 14 - Which of the following components is not involved...Ch. 14 - Which of the following does the enzyme primase...Ch. 14 - In which direction does DNA replication take...Ch. 14 - A scientist randomly mutates the DNA of a...Ch. 14 - The ends of the linear chromosomes are maintained...Ch. 14 - Which of the following is not a true statement...Ch. 14 - During proofreading, which of the following...Ch. 14 - The initial mechanism for repairing nucleotide...Ch. 14 - A scientist creates fruit fly larvae with a...Ch. 14 - Explain Griffith's transformation experiments What...Ch. 14 - Why were radioactive sulfur and phosphorous used...Ch. 14 - When Chargaffwas performing his experiments, the...Ch. 14 - Provide a brief summary of the Sanger sequencing...Ch. 14 - Describe the structure and complementary base...Ch. 14 - Prokaryotes have a single circular chromosome...Ch. 14 - How did the scientific community learn that DNA...Ch. 14 - Imagine the Meselson and Stahl experiments had...Ch. 14 - DNA replication is bidirectional and...Ch. 14 - What are Okazaki fragments and how they are...Ch. 14 - If the rate of replication in a particular...Ch. 14 - Explain the events taking place at the replication...Ch. 14 - What is the role of a primer in DNA replication?...Ch. 14 - Quinolone antibiotics treat bacterial infections...Ch. 14 - How do the linear chromosomes in eukaryotes ensure...Ch. 14 - What is the consequence of mutation of a mismatch...Ch. 14 - An adult with a history of tanning has his genome...
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- In making recombinant DNA, what is the benefit of using a restriction enzyme that cuts DNA in a staggered fashion?arrow_forwardA DNA fragment with 450 bp will be closer to the top (negative pole) of an electrophoresis gel than one with 2,500 bp.True or false?arrow_forwardWhat is generated from the replication of DNA ? what method is used ? Describe the process. What are Okazaki fragments? What enzymes are used ?arrow_forward
- Why do we need to use salt solution when we cloning dna?arrow_forwardWhy does DNA replication need to be error-free? Why does the DNA need to be a “careful reader?”arrow_forwardIn gel electrophoresis, are the DNA fragments attracted to, or repelled from the negative pole of the electric field? The positive pole? Why are these attractions and repulsions important to the process?arrow_forward
- What is an Okazaki fragment? In which strand of replicating DNA are Okazaki fragments found? Based on the properties of DNA polymerase, why is it necessary to make these fragments?arrow_forwardWhen proteins are separated using native gel electrophoresis, size, shape, and charge control their rate of migration on the gel. Why does DNA separate based on size, and why do we not worry much about shape or charge?arrow_forwardIs there any situation in which DNA is made based on a RNA template? What is the enzyme involved?arrow_forward
- DNase is an endonuclease that degrades DNA. Which steps or/and reagents in the DNA isolation procedure prevent endonucleases from degrading DNA?arrow_forwardWhat features of the structure of DNA enable it to be replicated?arrow_forwardExplain why the bending of the double helix to form a superhelix falls into the area of geometry or mathematics? How does Topoisomerase I relaxes the DNA supercoils?arrow_forward
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