1. Many dyes and pigments are organic molecules which vary in their relative polarities. Look up and draw the structures of two water-soluble dyes: malachite green (a.k.a. basic green 4) and rhodamine 6G (a.k.a. basic red 1).

Fundamentals Of Analytical Chemistry
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Chapter29: Mass Spectrometry
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Problem 29.12QAP
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I have the chemical structures drawn out. I am struggling with which are polar and why they are. Which would have the greatest Rfactor and why based on looking at molecular structure and your knowledge And if you added a non polar solvent to each repeatedly what would happen to the Rfactor.
1. Many dyes and pigments are organic molecules which vary in their relative polarities. Look
up and draw the structures of two water-soluble dyes: malachite green (a.k.a. basic green 4)
and rhodamine 6G (a.k.a. basic red 1).
2. If you ran a chromatogram comparing these two dyes with water as the solvent, which would
you expect to have a higher Rf value (travel further)? Why?
3. What would happen to the Rr values of these dyes if you ran several chromatograms using
solvents of decreasing polarity? Why?
Transcribed Image Text:1. Many dyes and pigments are organic molecules which vary in their relative polarities. Look up and draw the structures of two water-soluble dyes: malachite green (a.k.a. basic green 4) and rhodamine 6G (a.k.a. basic red 1). 2. If you ran a chromatogram comparing these two dyes with water as the solvent, which would you expect to have a higher Rf value (travel further)? Why? 3. What would happen to the Rr values of these dyes if you ran several chromatograms using solvents of decreasing polarity? Why?
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