1. The following are the TLC results from running compounds A and B using column chromatography. What is the best solvent system to elute compound A? How about for compound B? Provide the basis of your answer. O-O A B A B A B A B AB A B 3:1 4:1 6:1 81 10:1 12:1 hexanes'ethyl acetate
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- Use a suitable model to explain how separation and identification of a mixture of organic compounds can be achieved with a thin layer chromatographic (TLC) technique.Upon screening with TLC (made of silica gel) chromatography using hexane/chloroform (3:1) as eluting solution, 3 spotswere detected corresponding to a (A) polyphenol, a (B) tannin and an (C) alkaloid. Write the letters of the answer onthe spots in the chromatogram shown at the picture.You spotted a mixture of three compounds, labeled A-C, on a silica plate. The resulting thin layer chromatography (TLC) plate with a mobile solvent mixture of 95 % hexanes and 5 % ethyl acetate is shown below. Which of the spots traveled the furthest up the TLC plate corresponding to spot 3?
- A thin-layer chromatography (TLC) experiement was run using three compunds: benzophenone, benzhydrol, and biphenyl. The three solvents that were used with the three compounds were hexane, ethyl acetate, and dichloromethane. Which solvent would be the best for separating these compounds and why?The three TLC plates below show the analysis of the same mixture of two compounds. One plate was developed in 100% DCM, one in 90:10 DCM/MeOH, and one in 80:20 DCM/MeOH. Determine which plate was developed in which solvent.1) When you try separating an unknown mixture by normal-phase liquid chromatography with 50% hexane and 50% methyl t-butyl ether, the peaks are too close together. (not resolved) Should you use a higher or lower concentration of hexane in the next run?
- Upon screening with TLC (made of silica gel) chromatography using hexane/chloroform (3:1) as eluting solution, 3 spots were detected corresponding to a (A) polyphenol, a (B) tannin and an (C) alkaloid. Write the letters of your answers on the spots in the chromatogram shown at the right.While preparing the mobile phase to run a monoamine neurochemical HPLC analysis, you miscalculated the amount of acetonitrile you needed to add to your aqueous solution and came up with a 20% acetonitrile solution. How would this small mistake affect the resulting chromatographs?Explain how TLC and column chromatography can be used together to ensure the perfect separation of two organic compounds
- You were analyzing a mixture of volatile solvents using Gas Chromatography and found out that two peaks are unresolved (not well-separated) using your initial program. Which method can you use to obtain a chromatogram with well separated peaks?A thin-layer chromatography (TLC) experiment was run using three compounds: benzophenone, benzhydrol, and biphenyl. The three solvents that were used with these compounds were hexane, ethyl acetate, and dichloromethane. Which solvent would be the best for separating these compounds and why?Which of the solvents below can be used with reversed phase chromatography? a. Acetonitrile b. Toluene c. Hexane d. Carbon tetrachloride