pon screening with TLC (made of silica gel) chromatography using hexane/chloroform (3:1) as eluting solution, 3 spots were detected corresponding to a (A) polyphenol, a (B) tannin and an (C) alkaloid. Write the letters of the answer on
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Upon screening with TLC (made of silica gel) chromatography using hexane/chloroform (3:1) as eluting solution, 3 spots
were detected corresponding to a (A) polyphenol, a (B) tannin and an (C) alkaloid. Write the letters of the answer on
the spots in the chromatogram shown at the picture.
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- Upon screening with TLC (made of silica gel) chromatography using hexane/chloroform (3:1) as eluting solution, 3 spots were detected corresponding to a (A) polyphenol, a (B) tannin and an (C) alkaloid. Write the letters of your answers on the spots in the chromatogram shown at the right.Upon screening with TLC (made of silica gel) chromatography using hexane/chloroform (3:1) as eluting solution, 3 spotswere detected corresponding to a (A) polyphenol, a (B) tannin and an (C) alkaloid. If the TLC chromatogram in the picture was duplicated but using a column, draw the set-up and label approximate spots.Upon screening with TLC (made of silica gel) chromatography using hexane/chloroform (3:1) as eluting solution, 3 spots were detected corresponding to a (A) polyphenol, a (B) tannin and an (C) alkaloid. Write the letters of the on the spots in the chromatogram shown at the right.
- Amino acids and fatty acids do not readily elute from gas chromatography columns even at temperatures above 200 degrees Celsius. What can be done to these biomolecules to allow gas chromatographic analysis?Use a suitable model to explain how separation and identification of a mixture of organic compounds can be achieved with a thin layer chromatographic (TLC) technique.6.0 mL of cyclohexanol, 8 mL of 85% phosphoric acid, and 10 drops of sulfuric acid are added to a 50-mL round-bottom flask with boiling chips, and the mixture is distilled into an ice-cooled receiver. The distillate is in the form of a colorless bi-phasic mixture. The cyclohexene upper layer is removed from the water layer, and dried over anhydrous calcium chloride. Isolated is cyclohexene as a clear and colorless foul-smelling liquid. Weighing data is given below. Gross mass: 51.24 g Tare mass: 48.04 g Product mass: 3.2g Question: Calculate the theoretical mass of the cyclohexene product, bearing in mind that the cyclohexanol starting material was measured by volume, not by mass (so you must use the density of cyclohexanol to convert milliliters to grams, before calculating the number of moles). Calculate the percent yield of cyclohexene obtained.
- 2-pentanone has a retention index of 987 on a poly(ethylene glycol) column (also called Carbowax). (a) Between which two straight-chain hydrocarbons is 2-pentanone eluted? (b) An unretained solute is eluted from a certain column in 1.80 min. Decane (C10H22) is eluted in 15.63 min and undecane (C11H24) is eluted in 17.22 min. What is the retention time of a compound whose retention index is 1 050?An unretained solute is eluted from a GC column in 1.80 min. Decane (C10H22) is eluted in 15.63 min and undecan (C11H24) is eluted in 17.22 min. What is the retention time (report to 2 decimal places, in minutes) of a compound whose retention index is 1050?Based on the resulting chromatograms after visualization with iodine vapor, which between the solvent systems WHITE and MIX resulted in a more effective separation of the pigments of the indigo extract? Explain your answer in terms of the solvent that did not effectively separate the components of the root extract.
- From the six analytes listed below, n-octanal, n-octane, 1-chlorooctane, n-octanol, n-heptane, and n-octanoic acid predict retention order (shortest to longest retention times) if the SP is: 100% polyethylene glycol or 100% dimethylpolysiloxane.During an assay of paracetamol by UV an analyst prepares a series of calibration solutions containing approximately 3-15 mcg/mL paracetamol as follows: 140.1 mg of pure paracetamol was added to a 200 mL volumetric flask. 0.1 M NaOH (50 mL) was added, the mixture well shaken and made up to volume with deionized water 10 mL of the above solution was diluted to 100 mL 1)What is the concentration of the final diluted solution in mcg/mL?(1 decimal) 2) What volume of the diluted solution is required to prepare 100 mL of a 8 mcg/mL standard solution? (1 decimal)1) When you try separating an unknown mixture by normal-phase liquid chromatography with 50% hexane and 50% methyl t-butyl ether, the peaks are too close together. (not resolved) Should you use a higher or lower concentration of hexane in the next run?
