1. The following data are for a liquid chromatographic column:length of packing: 24.7 cmVM : 1.37 mLFlow rate: 0.313 mL /minVs: 0.164 mlA chromatogram of a mixture of species A, B, C, and D provided the following data:Retention time, min3.15.4Width of Peak Base (W), minNonretained13.314.121.60.411.071.161.72Calculate: (a) the number of plates from each peak.(b) the mean and standard deviation for N.(c) the plate height for the column.(d) the capacity factor for A, B, C, and D.(e) the resolution for species B and C.(f) the length of column necessary to separate species C and D with a resolution of 1.5.ABCD

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1. The following data are for a liquid chromatographic column: length of packing: 24.7 cm VM: 1.37 mL Flow rate: 0.313 mL /min Vs: 0.164 ml A chromatogram of a mixture of species A, B, C, and D provided the following data: Retention time, min Width of Peak Base (W), min Nonretained 3.1 5.4 0.41 1.07 1.16 1.72 13.3 14.1 21.6 Calculate: (a) the number of plates from each peak. (b) the mean and standard deviation for N. (c) the plate height for the column. (d) the capacity factor for A, B, C, and D. (e) the resolution for species B and C. (f) the length of column necessary to separate species C and D with a resolution of 1.5. ABCD

1. The following data are for a liquid chromatographic column: length of packing: 24.7 cm VM: 1.37 mL Flow rate: 0.313 mL /min Vs: 0.164 ml A chromatogram of a mixture of species A, B, C, and D provided the following data: Retention time, min Width of Peak Base (W), min Nonretained 3.1 5.4 0.41 1.07 1.16 1.72 13.3 14.1 21.6 Calculate: (a) the number of plates from each peak. (b) the mean and standard deviation for N. (c) the plate height for the column. (d) the capacity factor for A, B, C, and D. (e) the resolution for species B and C. (f) the length of column necessary to separate species C and D with a resolution of 1.5. ABCD

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter26: An Introduction To Chromatographic Separations

Section: Chapter Questions

Problem 26.14QAP

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