1. Upon the banana DNA extraction, what do you see in the top portion of the liquid when you look at your container?2. Is the DNA you extracted pure? What are the possible impurities?3. What can we do with the DNA once we have purified it? Discuss different techniques and technologies associated with this. III. Procedure:1. First you will need to put the 2 banana and 4 cup distilled water in the plasticbag, seal the bag and mash them to make your slurry.2. In the plastic cup, mix a solution of 1 tsp. shampoo, 2 pinches of salt and 4 tsp.distilled water. Stir this solution slowly for about a minute until the shampoodissolves in the water.3. Now you will add 2 tsp. of the banana slurry to the soap solution and stir for fiveminutes.4. Next, make a well with the coffee filter and place it in the empty cup. Do not letthe filter touch the bottom of the cup. Pour in the liquid mixture and let it filter.5. Fill the narrow tube with 2 tsp. of cold isopropyl alcohol. Very slowly, add 2 tsp.of the filtered banana mixture so that there are two layers of liquid.6. Let the tube sit for 2-3 minutes without disturbing the solution. You will see theclear/white DNA precipitate into the alcohol layer.7. If you wish to keep the DNA, remove it using a bamboo skewer (a twirlingmotion works best) and place it in a capped tube filled with alcohol. IV. Guide Questions:Explain your answer and cite references in APA format.1. What does mashing do to the fruit?2. Why did you add detergents?3. What do you think the ethanol does? Why can't we use room temperature ethanol?4. To extract DNA from cells, what must you isolate it from in the case of a plant such asstrawberry?5. Look at your container, what do you see in the top portion of the liquid?6. Is the DNA you extracted is pure? What are the possible impurities?7. What can we do with the DNA once we have purified it? Discuss different techniquesand technologies associated with this.8. Imagine that there is an E. coli outbreak in your area, and you would like to test thekangkong from your local grocery store. How could you modify this protocol toextract DNA from the kangkong (to identify the species) and check for presence orabsence of E. coli.? Keep in mind that (i) E. coli is free-living and not an endosymbiont,and (ii) plant cells are encased in both a cell membrane and cell wall.

DNA (Deoxyribonucleic Acid) is the genetic material of all living organisms whether plant, animal,…

1. Upon the banana DNA extraction, what do you see in the top portion of the liquid when you look at your container? 2. Is the DNA you extracted pure? What are the possible impurities? 3. What can we do with the DNA once we have purified it? Discuss different techniques and technologies associated with this.

1. Upon the banana DNA extraction, what do you see in the top portion of the liquid when you look at your container? 2. Is the DNA you extracted pure? What are the possible impurities? 3. What can we do with the DNA once we have purified it? Discuss different techniques and technologies associated with this.

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter1: The Clinical Laboratory

Section1.8: Laboratory Math And Reagent Preparation

Problem 2.2CS

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Procedure: 1. Prepare 5 test tubes. Place 1 ml of 1% starch and add 10 drops of saliva to each tube. Mix thoroughly. 2. Place the first tube in ice water, the 2nd tube leave at room temperature, the 3rd tube in 40°C , the 4th tube at 60°Cwater bath and the 5th tube boil for 2 minutes.. 3. Leave the 4 tubes in their respective temperatures for 30 minutes. The 4th tube allows to stand for 30 minutes after heating for 2 minutes. 4. Test the contents of each tube with iodine and benedict’s tests.
Procedure:1. Prepare 5 test tubes. Place 1 ml of 1% starch and add 10 drops of saliva toeach tube. Mix thoroughly.2. Place the first tube in ice water, the 2ndtube leave at room temperature, the 3rdtube in 40°C , the 4thtube at 60°Cwater bath and the 5thtube boil for 2minutes..3. Leave the 4 tubes in their respective temperatures for 30 minutes. The 4thtubeallows to stand for 30 minutes after heating for 2 minutes.4. Test the contents of each tube with iodine and benedict’s tests.
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