4. The image below is based on the Meselson Stahl experiment where bacteria are grown in 15N and movedto 14N.Please match the tube in the image to the descriptors a-ea) DNA from E. coli grown in 15Ntest tube 1test tube 2test tube 3test tube 4test tube 5b) DNA from E. coli grown in 14Nc) A 1:1 mixture of DNA from cellsgrown in 14N and cells grown in 15Nd) A 1:1 mixture of DNA grown in 14Nand 15N, heated and allowed to re-anneale) DNA containing one strand of 15Nand one strand of 14N

The Meselson-Stahl experiment showed that DNA replication is a semiconservative process. In this…

Answered: 4. The image below is based on the…

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter17: Bacterial And Viral Genetics

Section: Chapter Questions

Problem 1TYK

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The cath sample was both filtered to remove all bacteria, and then treated with DNase. Based on what you know about the cath sample contents, how did the bacteria exposed to this treated sample still become antibiotic resistant?
1. Scientists were able to develop transgenic bacteria that can be used for mercury remediation. This process involved combining genes for mercury resistance (so that the bacteria itself has protection) and mercury accumulation (thereby allowing the bacteria to sequester Hg for retrieval later on). They probably used the following techniques in creating the transgenic bacteria EXCEPT a. plasmids b. DNA ligase c. DNA polymerase d. restriction enzymes 2. Consider these two parents (Mom and Dad) and their son, Calvin. (see pic attached). Which two members of this family would show the greatest difference in their DNA profile?
1) Which technique is best suited to determining which genes are activated in a bacterium during infection while causing disease in a person. a) SDS-page b) microarray analysis c) RFLP analysis d) clone library analysis 2)Which of the following is not an application of PCR? a) Determine if two people are related. b) Identify a bacterial pathogen in a patient sample. c) Determine the gene sequence of the gene that codes for a bacterial enterotoxin. d) These are all applications of PCR.
you have transformed E.coli cells with a plasmid containing the gene for the enzyme beta-lactamase you have used 3µl of a stock solution of 2.5ng/µl DNA. You transformed 150/µl of competent cells, using the calcium chloride method. you have added 600µl of luria broth to the cells and then plated out 200µl on triplicate plates. upon counting the colonies, you got the following results: plate 1: 120 cfu plate 2: 35 cfu plate 3: 95 cfu please answer the following question: 1. what new characteristic will the cells have ater transformation? 2. how are you going to test for the new characteristic? 3. calculate the transformation efficiency of the experiment?
1. Briefly explain why the total size of the pMBBS plasmid in the Restriction Enzymes practical is 3000bp (base pairs) 2. Briefly explain why the cut sites on the pMBBS plasmid in each Restriction Enzymes (EcoRI, BamHI, and XhoI) just 1 each 3. Briefly explain what led to the 5 fragments formed which linked to the 500bp, 1000bp, 1500bp, 2000bp, 2500bp sizes
Write a result paragraph of the isolation and purification of bacteriophage with E. coli Top10 with the following results: At 10^-1 to 10^-3 no plaques were formed. At 10^-4 too numerous plaques were formed. At 10^-5, 10^-6 and 10^-7 countable plates of 22, 7 and 1 respectively. At 10^-8 and 10^-9 no plaques formed but present of host bacteria.
What is PCR? Why does Taq polymerase work better than a typical DNA Polymerase isolated from E. coli for PCR? The optimal growth temperature for E Coli is 37 °C.
In the "Bacterial Transformation" experiment, why were there no fluorescence bacteria present in the other plates even though these were inserted by the +pGLO plasmid? (Note: Discuss the importance on the addition of ampicillin and arabinose to the medium of the genetically engineered bacteria). Limit your answer to 1-3 sentences only.
Researchers are designing several experiments to test the ability of Salmonella bacteria to develop antibiotic resistance. A culture of Salmonella bacteria is exposed to the same concentrations (200 mg/L) of an antibiotic for four days. The table shows the number of isolated resistant bacteria over a four-day period. Which of the following statements best explains these results? A - The bacteria were not affected by the antibiotic. B - After being exposed to the antibiotic, the bacteria altered their DNA. C - A new species of bacteria emerged after the antibiotics were introduced. D - Random mutations led some bacteria to be resistant and, over time, they increased in the population.
In the Avery, McCarty and McCleod experiment, DNA was identified as the heritable material because, A. R bacteria, when precipitated out of the test tube with an antibody, gave rise to bacterial colonies on a nutrient agar plate. B. R bacteria, when precipitated out of the test tube with DNase, gave rise to bacterial colonies on a nutrient agar plate. C. The transforming principle was susceptible to treatment with protease D. S bacteria were not precipitated out of the test tube by an anti-R bacteria antibody when S bacterial extracts were incubated with DNase E. S bacteria were not precipitated out of the test tube by an anti-R bacteria antibody when S bacterial extracts were incubated with protease
Is the DNA you extracted is pure? What are the possible impurities? What can we do with the DNA once we have purified it? Discuss different techniques and technologies associated with this. Imagine that there is an E. coli outbreak in your area, and you would like to test the kangkong from your local grocery store. How could you modify this protocol to extract DNA from the kangkong (to identify the species) and check for presence or absence of E. coli.? Keep in mind that (i) E. coli is free-living and not an endosymbiont, and (ii) plant cells are encased in both a cell membrane and cell wall.
what are the plasmid status of bacterial cells resulting from conjugation between a f+ and a f- bacterium ? * a-Two F+ bacteria b-Two F- bacteria c-The F+ bacterium become F- and the F- bacterium become F+ d- The F+ bacterium remain as F+ , and the F- bacterium remain as F-

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