5. Below is an image of DNA sequenced using the Sanger sequencing method, where different nucleotides arelabeled with different colors.Top of gel(shortest or longestDNA fragment?)a) Label the shortest and longest fragments on the gel by filling out theblank next to the image.ddGTPLabeled terminatorddCTPNucleotides used inddTTPsequencing reactionddATPb) Determine the sequence of the original gene (hint: remember that thesequence is complimentary to the original gene because it is copied by PCR(via base pairing) prior to loading the gel).Gel sequence:Original Gene:+ (shortest or longestDNA fragment?)

Gel electrophoresis is a molecular technique that aims to separate the DNA, RNA, and protein…

5. Below is an image of DNA sequenced using the Sanger sequencing method, where different nucleotides are labeled with different colors. Top of gel (shortest or longest DNA fragment?) a) Label the shortest and longest fragments on the gel by filling out the blank next to the image. ddGTP Labeled terminato Nucleotides used sequencing reactie ddCTP dLIPP ddATP b) Determine the sequence of the original gene (hint: remember that the sequence is complimentary to the original gene because it is copied by PCR (via base pairing) prior to loading the gel). Gel sequence:

5. Below is an image of DNA sequenced using the Sanger sequencing method, where different nucleotides are labeled with different colors. Top of gel (shortest or longest DNA fragment?) a) Label the shortest and longest fragments on the gel by filling out the blank next to the image. ddGTP Labeled terminato Nucleotides used sequencing reactie ddCTP dLIPP ddATP b) Determine the sequence of the original gene (hint: remember that the sequence is complimentary to the original gene because it is copied by PCR (via base pairing) prior to loading the gel). Gel sequence:

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter19: Genomes And Proteomes

Section: Chapter Questions

Problem 1TYK

See similar textbooks

Similar questions

What information and materials are needed to amplify region of DNA using PCR?
After a polymerase chain reaction (PCR), agarose gel electrophoresis is often used to: a. amplify the DNA. b. convert cDNA into genomic DNA. c. convert cDNA into messenger RNA. d. verify that the desired DNA sequence has been amplified. e. synthesize primer DNA molecules.
The experiments in which Meselson and Stahl grew bacteria in heavy nitrogen conclusively demonstrated that DNA (a) is a double helix (b) replicates semiconservatively (c) consists of repeating nucleotide subunits (d) has complementary base pairing (e) is always synthesized in the 5 3 direction
The results of a paternity test using short tandem repeats are listed in the table below. Whos the daddy? How sure are you?
1. Which of the following are generated due to the twisting of dsDNA? a. Hydrophobic bonds b. Van der Waal forces c. Hydrogen bonds d. Grooves 2. Which of the following terms refers to the annealing of ssDNA to complementary RNA? a. Homologous combination b. Hybridization c. Denaturation d. Telomere lengthening 3. Which of the following completes this statement? The melting temperature of DNA is the temperature at which__________. a. DNA denatures b. DNA bonds are reinforced c. Solid DNA becomes liquid d. Liquid DNA coils
3. Using the same template strand, transcribe the DNA:GCA TAG CAA TGC
1. Which of the following actions in the DNA extraction of strawberry separates the DNA from the rest of the solution? a. Addition of cold isopropyl alcohol b. crushing the strawberry c. Addition of soapy salt solution d. Filtration of the crushed strawberries 2. What does the salt in the soapy salt solution do in the DNA extraction? a. It dissolves the DNA. b. It displaced a lot of proteins to free the DNA. c. It disrupts the phospholipid bilayer. d. It precipitates the DNA.
1. What is/are the purpose(s) of DNA extraction? 2. What is the function of each reagent used in the extraction of DNA from a banana? a. Saltwater b. Liquid dishwashing soap c. Cold isopropyl alcohol
4. What is the name of the process by which bacteria pick up a different organism’s geneticmaterial?5. Genetically, how does the original bacterial DNA (plasmid) differ from the final bacterial DNAmolecule? (Do not say, “It is longer.”)6. Tetracycline is an antibiotic that is prescribed to kill bacterial infections. The transformedbacterial cell that you created has the tetracycline resistant gene in it. If this cell is placed on agrowth medium with tetracycline, will the bacteria grow or die? Explain.
1- The following Wild Type DNA codes for color in a lizard living in the Namib Desert (tan sand) a skin colorof reddish brown. This lizard is preyed upon by hawks flying above.Wild Type (Normal) DNADNA Template Strand: TAC-TTC-AAA-CCG-ATTDNA (silent strand): ATG-AAG-TTT-GGC-TAA For number 7, compare this change to the wild type in number 1. Remember that the originalpigment is a different color.7. Let us assume that the following protein (Met-Lys-Phe-Ser) changes the pigment deposited inthe lizard skin to a tan color. Would this be detrimental? Would this be beneficial? Explaineither answer.
1) Which statement below explains the trick in sanger sequencing that produces fluorescently labeled fragments at every length within a fragment? a) When synthesizing a copy of the DNA to be sequenced, a high concentration of fluorescently labeled dideoxynucleotides (ddNTPs) are used along with a low concentration of deoxynucleotides (dNTPs) to produce the chain termination events at every location in the sequence. b) When synthesizing a copy of the DNA to be sequenced, fluorescently labeled dideoxynucleotides (ddNTPs) are used instead of deoxynucleotides (dNTPs) to produce the chain termination events at every location in the sequence. c) When synthesizing a copy of the DNA to be sequenced, a low concentration of fluorescently labeled dideoxynucleotides (ddNTPs) are used along with a high concentration of deoxynucleotides (dNTPs) to produce the chain termination events at every location in the sequence. d) When synthesizing a copy of the DNA to be sequenced, fluorescently labeled…
1) What happened to the DNA at the different temperatures? How does Polymerase Chain Reaction exploit this property of DNA (Word count: 200) 2) Briefly outline the steps of DNA extraction as carried out in a lab for the purposes of sequencing, with reference to a published protocol from a company that supplies regents and kits for DNA extraction (e.g Qiagen, ThermoFischer, Invitrogen, or similar). Please Include the weblink for the published protocol you used as a reference