General CHEMISTRY one.. Lab 8 ( Food Dye Forensic) Please help me answer these questions Experiment 8Food Dye ForensicsTheory:By examining the ultraviolet-visible spectrum of a solution with a distinct color (or thatabsorbs light in the ultraviolet region), the wavelength of absorbed light and the energyassociated with the electronic transition can be determined. Wavelength and energy areinversely related to one another as seen in equation 1 below; h represents Plank's constant,6.626*10-34 J*s; c represents the speed of light, 3*108 m/s;represents wavelength, expressedin meters.hcE =(1)Small wavelengths such as gamma rays (some sources include nuclear radiation and thesun) are extremely dangerous and high in energy.penetrate the skin and even begin unfolding DNA, which can lead to cancer! However,wavelengths that are much larger, such as radio waves, can be beamed from local radio stationswithout causing any damage to humans due to their low energy. Have you ever wonderedwhat the radio station 92.3 means? The radio station is beaming a frequency of 92.3 MHz fromare so dangerous that they cantheir broadcasting tower. Just as energy and wavelength are related to one another, so isfrequency, represented bysmaller the wavelength. Frequency is measured in units of Hertz which is synonymous withinverse seconds (s-1).and wavelength, equation 2. The larger the frequency: theC = Av(2)So, what gives rise to the wavelength of light and how does spectrophotometry work? Ifyou place a sample of food dye in a cuvette (Figure 2), made of quartz, and then shine a lightthrough the sample, electronic transitions will occur in the molecules of each of the food dyes.Some of the light will be absorbed by the molecules (this is what causes the electronictransitions) and some of the light will pass through the sample and make it to the detector.Using this information,incident radiation from the light source. Based on the maximum(s) achieved from the graph,the wavelength(s) of absorption of the solution can be determined. FD&C Red 40 absorbs lightat approximately 500 nm, which can be determined from the top of the peak referred to asmax, as shown in Figure 1.of the sample can be plotted vs. the wavelength of the41 Post Lab Questions:1. Using the graphs of the food dyes created from your data, use the Imax to determine theamount of energy required for the electronic transition in J/photon and kJ/mole.2. Define the word excitation in the context of today's lab.3. The molar absorptivity,absorbance of a solution was found to be 0.75 and the pathlength of the cuvette usedwas 10 mm. What is the concentration of the solution in units of molarity?of a particular compound is 5,674 M-1*cm1. TheVasteono4. Solution A absorbs light at a wavelength of 525 nm. If solution B requires a largeramount of energy to absorb light, is the wavelength of solution B larger or smallercompared to solution A?5. Based on what you have learned in the lab today, how do you think suntan lotion worksto protect your skin from ultraviolet radiation? (Yes, you may use google to help youanswer this question! Please include the website if you do use an external source.)Dianeb onlo 1odmor boc47

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