9. Describe two biochemical tests in which you could help identify a bacterium in the lab. In your answer include the test name, the purpose of the test, and the start and end results of the test. (You can draw a picture if that is easier).
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Q: What is the purpose of an autoclave and what is its mechanism
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Q: more
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Q: 2. you do the Kirby-Bauer test on a hypothetical Staphylococcus species with penicillin and…
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Q: 5. To figure out the initial concentration of a bacterial culture, you made an 8 fold dilution of…
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Q: hat is a pure culture, and why is it important in microbiology?
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Q: 12. A student makes a smear by applying a bacteria onto the slide. A simple stain is performed, but…
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Q: 9. Other than bacteria list the other 6 types of Microorganisms listed in your textbook.asap
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- 4. All the following can be autoclaved EXCEPT: a) Biological material b) Glass ware c) Radioactive material d) Broth and gel media for growing bacteria5. The autoclave raises the atmospheric pressure to _____ : a) 10 psi b) 15 psi c) 20 k joules d) 25 watts6. In the experiment with serial dilutions, the plates which should be used for the final count for colonies should have a range of _______ CFU in order to have a reliable estimate: a) <10 b)10-15 c) 15-20 d) none of the above1. What is the most common sterilization technique used in laboratories? 2. List at least 5 procedures of the aseptic technique and describe its uses. 3. Why is Gram stain one of the most important and widely used stains in bacteriology? 4. Explain the Gram staining technique in chronological order. Indicate the reagent used and the time of usage on each reagent.2. You use tubes to test aerotolerance of bacteria. From your samples you have 3 results: A. Bacteria growing on the surface. B. Bacteria growing throughout the tube, the agar shows cracks. C. Bacteria growing about 5 mm below the surface. Please interpret each bacterial result. (Give the bacteria an oxygen classification, explain what classification means and interpret the cracks in the agar.)
- 1. What is the importance of using freshly prepared KOH in testing the bacteria? Explain this comprehensively and please, do not just copy from somewhere.1. When performing a bacterial lab, what could happen that could lead to incorrect results (potential pitfalls during the lab?)1)What is the natural color of cytoplasm? How is it related to our need to stain bacterial cells? 2) Which of the following is the main goal when staining a culture? A) improve contrast B) identify bacteria C) Visualize bacterial structures
- 4) In Gram Stain the bacteria that becomes colorless after washing with alcohol or acetone is the: a) Gram +ve bacteria b) Gram -ve bacteria c) ) Gram neutral bacteria d) archaebacteria e) b & c5) Antimetabolites include: a) Sulfonamides b) Tetracyclins c) Trimethoprim d) all of the above e) a and c6) Oil immersion is used in light microscope because: a) it increases only b) it decreases only c) it increases the / n sin value d) it decreases / n sin e) same refractive index as the glass slide2) A pure culture A) is sterile. B) is a population of identical cells. C) is made of a clearly defined chemical medium. D) contains one microbial cell.1. Which of the following is NOT true of spread plating method? A. the maximum inoculum allowed is 1.0 ml B. spreading of the inoculum is done using a disinfected glass L-rod C. A pre-solidified medium is required prior to inoculation of the sample. D. A and B 2. Plates inoculated with bacteria are inverted during incubation to: A. prevent contamination B. limit the colony growth C. A and B D. A and C E. All of the above F. None of the above
- 1. In Microbiology, are there technologies that can help make bacterial culture and sensitivity done faster?1. Why do you think the 5% sheep blood agar was selected for the culture of mouth bacteria?1. why is there a need to incubate cultures at room temperature? How can this affect microbes? 2. Explain why an inverted image is seen under a compound microscope.
