Matching Question:Researchers characterized a new enzyme and determined its Km=200 uM. Moreover, they found that at (Etotad10 nM this enzyme reaches maximum reaction velocity Venasa5 is1. What is the keat of this enzyme?2. At which concentration of the substrate this enzyme reaches initial reaction velocity (Vo) of 500 nM/s (assuming (Etotal is the same 10 nMy?3. What is the maximum reaction velocity (Vmax) of the reaction catalyzed by this enzyme at (Etotail4 nM?Make sure to choose the correct units of measure. Note that some of the items from the answer list should NOT be used.v kcat1. 400 uM- (S] -- Vmax2 100 uM500 s450 uM250 s1* 5017. so00 s1 UM'sSuMis10 200 uM11. 10 uMis12 25 s13. 500 nM/s

Given, Km = 200 μM [ETotal] = 10 nm = 0.01 Vmax = 2.5 μM/s

1. What is the keat of this enzyme? 2. At which concentration of the substrate this enzyme reaches initial reaction velocity (Vo) of 500 nMis (assuming (otal is the same 10 nMy? 3. What is the maximum reaction velocity (Vmax) of the reaction catalyzed by this enzyme at (Etotail4 nM? of the items from the answer list should NOT be used.

1. What is the keat of this enzyme? 2. At which concentration of the substrate this enzyme reaches initial reaction velocity (Vo) of 500 nMis (assuming (otal is the same 10 nMy? 3. What is the maximum reaction velocity (Vmax) of the reaction catalyzed by this enzyme at (Etotail4 nM? of the items from the answer list should NOT be used.

Biochemistry

9th Edition

ISBN:9781305961135

Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Chapter6: The Behavior Of Proteins: Enzymes

Section: Chapter Questions

Problem 31RE: MATHEMATICAL You do an enzyme kinetic experiment and calculate a Vmax of 100 mol of product per...

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