[S] << Km [S] = = Km [S] >> Km Not true for any of these conditions Almost all active sites are empty. [ES] is much higher than [Efree]. [Efree] is equal to [ES]. Reaction rate is independent of [S. [Efree] is about equal to [Etotai]- Increasing [Etotal] will lower Km-

[S] << Km [S] = = Km [S] >> Km Not true for any of these conditions Almost all active sites are empty. [ES] is much higher than [Efree]. [Efree] is equal to [ES]. Reaction rate is independent of [S. [Efree] is about equal to [Etotai]- Increasing [Etotal] will lower Km-

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter27: Metabolic Integration And Organ Specialization

Section: Chapter Questions

Problem 20P: Figure 27.3 illustrates the response of R (ATP-regenerating) and U (ATP-utilizing) enzymes to energy...

See similar textbooks

Related questions

Q: 10. For the situations described below, indicate whether KM , Vmax, both parameters, or neither para...

A: The parameters KM and Vmax will show different alterations according to the situations described bel...

Q: Aberrant signaling through the Ras-BRaf-MAPK signal transduction pathway drives many cancers. This m...

A: Ras-BRaf-MAPK signal transduction pathway is well characterized pathway and known to transduce extra...

Q: Is this the correct answer of finding what the charge is?

A: Amino acids are the smallest units that make up the peptides or proteins inside the cell. Twenty sta...

Q: My question is Explain why you feel numbness when using a topically applied pain cream that cont...

A: Lidocaine is an an anesthetic and it leads to numbness of that particular area in which it is applie...

Q: 1. in cherry crandall method for lipase analysis what is the substrate used?

A: As you have asked multiple questions, we would solve first question for you. If you specific questio...

Q: 2. which of the following is the reason why hemolysis shoule be avoided? a. enzymes have lower con...

A: Q2. Hemolysis is defined as the rupture of erythrocyte membranes, which results in the release of ha...

Q: Double bonds are the sites of clouds of ________, making them easy targets in lipid oxidation. fill...

A: Chemical bonds can be classified into covalent, ionic and dative bonds. The C-C (Carbon -Carbon) bon...

Q: Titration of alanine by a strong base, for example NaOH, reveals two pk's. Which is the titration re...

A: A titration curve of an amino acid is the plot of the pH of the amino acid solution versus the volum...

Q: Net filtration pressure is calculated by ________.a. adding the capillary hydrostatic pressure to th...

A: During filtration, the pressure forces the fluid and all the solutes that appear smaller through the...

Q: How to use the Kd to determine the fraction of both bound proteins? The Kd is 0.05uM. protein 1 has ...

A: In the question following quantity has been given Kd=0.05uM [P1]= 0.03 uM [P2]=0.05uM

Q: electron transport chain, yet somehow iron is utilized as an electron carrier multiple times. Choose...

A: Electron transport When the electrons pass into the electron transport chain through reoxidation of ...

Q: According to the diagram, what is the average net charge of this amino acid at pH = 11.5? %3D Titrat...

A: A titration curve of an amino acid is the plot of the pH of the amino acid solution plotted against ...

Q: Balance the chemical equation for the oxidation of arachidonic acid to ATP, CO2, and water. (Use the...

A: In the question given, Arachidonic acid is oxidized to ATP CO2 and water. Fatty acid oxidation proce...

Q: Does fructose can produce a positive result in Seliwanoff Test? Elaborate your answer

A: Seliwanoff’s test is a test for carbohydrates that can distinguish between aldose and ketose sugars....

Q: we need to get the three pka values of Arginine on this graph so we need the pka (COOH) pka( NH3+) p...

A: Within the usual aqueous pH range, triprotic amino acids have an extra group as part of their variab...

Q: What are different kind of forces present in primary , secondary and Tertiary structure of protein ....

A: Amino acids are the basic structural and functional units of proteins. They are the monomeric units ...

Q: 1.) What are the main structural features of the mucupolysaccharides chitin? 2.) How do this aid in ...

A: Chitin is a carbohydrates polymer that is composed of N-acetyl glucosamine units attached together v...

Q: . Glycosidic bond between galactose and glucose in lactose is ______. In 2-3 sentences, explain why ...

A: Carbohydrates are composed of carbon, oxygen, and hydrogen which are connected by the glyco...

Q: Please help me with this. *Also Please Note: This is ONE problem set and according to the policy, a...

A: Note : Hi ! Thank you for the question. We are authorized to answer three subparts at a time. Since ...

Q: A corn geneticist wants to obtain a corn plant that hasthe three dominant phenotypes: anthocyanin (A...

A: Cross over: It is important for the normal segregation of chromosomes during meiosis and to produce...

Q: Assume that mitochondria contain 0.23 Molar KCL and 0.010 Molar NaCl. Calculate the grams per liter,...

A: KCl will dissociate into K+ and Cl-. However, NaCl will dissociate into Na+ and Cl-. Therefore, both...

Q: A. Determine the standard Gibbs free energy of the reaction. B. In dying E. coli cells, the concentr...

A: Hexokinase is a initial glycolysis enzyme and rate limiting enzyme, catalyzes the phosphorylation of...

Q: Compute for the dilution factor of the WBC pipet if blood is sucked up to 0.2 mark and the diluting ...

A: WBC pipette has markings upto 1 where blood can be sucked and 11 is total capacity of pipette for di...

Q: 1. What is the major role of magnesium in the body? 2. Give the different method of magnesium deter...

A: Magnesium is the fourth most abundant cation in the body. A balance of magnesium is maintained by re...

Q: A technician investigated the effect of temperature on the rate of an enzyme- controlled reaction. A...

A: The substrate molecules and catalysts undergo distinct changes in the reaction when the enzyme funct...

Q: 5. What is the definition of hydrolosis reaction?

A: Hydrolysis equation: AB + H2O gives AH + BOH

Q: True or false: F1 unit of ATP synthase is capable of synthesizing 3ATP molecules per a complete rev...

A: The Electron Transport Chain is an orderly arrangement of electron carriers in the inner mitochondri...

Q: 1. Explain the circadian rhythm and diurnal variation in the levels of serum phosphate. 2. What rel...

A: Circadian rhythm in simplest terms is the body clock that we follow. It is a result of complex time ...

Q: The B pleated sheet secondary structure can be formed through either intramolecular hydrogen bonding...

Q: Examine the diagram to the right to complete the following questions. 9. Which letter (a, b, s or d)...

A: Ionic bond-It is the bond form by the transfer of electron from one atom termed as donor to a accept...

Q: Differentiate thymolphthalein monophosphate method from alpha naphthyl phosphate method of prostatic...

A: Prostatic acid phosphatase: It is a glycoprotein synthesized by the prostate gland that is capable ...

Q: ou have given two proteins with a pI of 4.5 and pI of 7.7? Using an anion-exchange column, how can t...

A: Ion-exchange chromatography: The method involves the separation of ions and polar molecules based o...

Q: Part I. Answer the following questions in 5 to 7 sentences. 1. What are pentoses? What are the roles...

A: Carbohydrates are also known as hydrates of carbon. They contain elements such as carbon, hydrogen a...

Q: calculate the filtered load of a substance that is dissolved in plasma given the plasma concentratio...

A: Filtered load - Total substance amount filtered in the Bowman's space per time unit. Plasma concentr...

Q: What are the main structural features of the polysaccharides cellulose? How do this aid in its funct...

A: Carbohydrates are classified into three types on the basis of number of sugar units they contain whi...

Q: sketch a graph with appropriate axis titles that demonstrate the connections between enzymes and act...

A: Enzymes are highly specialized proteins that have extraordinary catalytic power, greater than that ...

Q: Draw the Lock and Key and Induced-Fit Models of enzyme-substrate complex

A: The interaction between an enzyme and a substrate can take place following the lock and key or the i...

Q: 20. The process below is otherwise known as HC OC-(CH) CH, Fat +S H,C(CH)e- Na HC-O-H a. alkali degr...

A: Soaps are the sodium and potassium salts of the long-chain carboxylic acids. Some of the examples o...

Q: Which of the alternatives below indicates techniques used both to purify and to concentrate proteins...

A: Proteins are polymers of amino acids linked by peptide bond and it is one of the important biomolecu...

Q: Using a pipette, add 2 mL of suspended DEAE SEPHAROSE (anion) resin to a chromatographic column. Rep...

A: DEAE Sepharose matrix is used in Anion exchange column. In the given method power of salt strength i...

Q: True or false .Very powerful X-rays used in crystallography are generated in a very large facilities...

A: The answer of the following question is given below

Q: In 1973, Stanley Cohen and Herbert Boyer inserted a gene from an African clawed frog into a bacteriu...

A: Stanley Cohen and Herbert Boyer were the first introduce the genes from one living organism to anoth...

Q: Vasopressin or antidiuretic hormone (ADH) is a peptide hormone synthesize nypothalamus. This hormone...

A: Cyclic AMP is a cyclic nucleotide that is chemically known as 3'5'adenosine phosphate. It is synthes...

Q: To which class does each enzyme belong? Explain hexokinase alanine aminotransferase alcohol deh...

A: The enzymes are divided into 7 classes mainly oxidoreductases, transferases, hydrolases, ligases, ly...

Q: True / False: β-bends can link together α-helices and β-sheets.

A: Proteins are the end products of a decoding process that begins with cellular DNA information. Prote...

Q: What is the order of the electron transport chain starting from FADH2 and ending with Oxygen

A: The energy released during the oxidation of glucose to carbon dioxide generates NADH and FADH2. The...

Q: 1. How is the cell membrane similar to a plastic bag with tiny holes? 2. What two components make up...

A: Since there are multiple questions asked, we will solve only the first three questions. If you need ...

Q: 1. Maltose is a disaccharide of, Explain in 2-3 sentences why you chose this answer. A. glucose and ...

A: Disaccharides are formed when two monosaccharides are joined together and a molecule of water is rem...

Q: 1. How is the cell membrane similar to a plastic bag with tiny holes? 2. What two components make up...

A: The extracellular and intracellular environment is aqueous and polar. The cell membrane creates a hy...

Q: 5) In an experiment to investigate the inhibition of the enzyme-glucosidase the following data for t...

A: Lineweaver-Burk Plot: This plot is also called as double reciprocal plot as equation for the plot is...

Question

The Michaelis‑Menten equation models the hyperbolic relationship between [S] and the initial reaction rate ?0V0 for an enzyme‑catalyzed, single‑substrate reaction E+S↽−−⇀ES⟶E+PE+S↽−−⇀ES⟶E+P. The model can be more readily understood when comparing three conditions: [S]<<?m[S]<<Km, [S]=?m[S]=Km, and [S]>>?m[S]>>Km.

Match each statement with the condition that it describes.

Note that "rate" refers to initial velocity ?0V0 where steady state conditions are assumed. [Etotal][Etotal] refers to the total enzyme concentration and [Efree][Efree] refers to the concentration of free enzyme.

[S] << Km
[S] = Km
[S] >> Km
Not true for any of these
conditions
Almost all active sites are empty.
[ES] is much higher than [Efree].
[Efree] is equal to [ES].
Reaction rate is independent of
[S.
[Efree] is about equal to [Etotal].
Increasing [Etotal] will lower Km-
Answer Bank
Incorrect

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

Step 2

Step 3

Step 4

Step 5

Trending now

This is a popular solution!

Step by step

Solved in 5 steps

SEE SOLUTION Check out a sample Q&A here

Similar questions

Would you expect an “enzyme” designed to bind to its target substrate astightly as it binds the reaction transition state to show a rate enhancementover the uncatalyzed reaction? In other words, would such a protein actuallybe a catalyst? Explain why or why not.
At what substrate concentration would an enzyme with a kcat of 25.0 s-1 and a KM of 3.5 mM operate at 25% of its maximal rate? How many reactions would the enzyme catalyze in 45 seconds when it is fully saturated with substate, assuming the enzyme has one active site?
If you graph the velocity of an enzymecatalyzed reaction vs. [S] for each of two substrates that are partof a random mechanism, would you expect to see the same shapecurve? Why or why not?
Do you think the hydrolysis GTP and ATP are similar? Explain why or why not?
An enzyme contains an active site aspartic acid with a pKa = 5.0, whichacts as a general acid catalyst. On the accompanying template, draw thecurve of enzyme activity (reaction rate) versus pH for the enzyme (assumethat the protein is stably folded between pH 2–12 and that the active siteAsp is the only ionizable residue involved in catalysis). Briefly explain theshape of your curve.
In the scheme below which represents the mechanism of action for a large number of enzymes: A+B⟺AB⟶C The steady state approximation is reached when: d[AB]/dt≈0 k2≫k1 k−1≫k1 k−1=k1
A potent inhibitor effectively inhibits an enzyme catalyzed reaction. What kind of a Ki value you would expect for a potent inhibitor?
Consider the binding reaction L + R → LR, where L is a ligand and R is its receptor. When 1 × 10−3 M of L is added to a solution containing 5 × 10−2 M of R, 90 percent of the L binds to form LR. What is the Keq of this reaction? How will the Keq be affected by the addition of a protein that facilitates (catalyzes) this binding reaction? What is the dissociation equilibrium constant Kd?
When enzyme solutions are heated, there is a progessive loss of catalytic activty over time due to denaturation of the enzyme. A solution of the enzyme hexokinase incubated at 45 degrees Celsius lost 50% of its activity in 12 minutes, but when incubated at 45 degrees Celsius in the presence of a very large concentration of one of its substrates, it lost only 3% of its activity in 12 minutes. Suggest why thermal denaturation of hexokinase was retarded in the presence of one substrates.
A histidine side chain is known to play an import-ant role in the catalytic mechanism of an enzyme; how-ever, it is not clear whether histidine is required in its pro-tonated (charged) or unprotonated (uncharged) state. Toanswer this question you measure enzyme activity over arange of pH, with the results shown in Figure Q2–1. Whichform of histidine is required for enzyme activity?
If the higher value of KM resulting in the new plot ( red curb ) is due to the presence of an enzyme inhibitor is inhibitor reversible or irreversible? And why?
An enzyme catalyzes a reaction in which substrate A is cleaved into two products, P and Q. In the catalytic mechanism, the enzyme converts A to an covalently-bound reaction intermediate X and product P, P then desorbs from the enzyme, and in a second chemical step, the enzyme converts the intermediate X in the EX complex to the final product Q (in EQ), which then desorbs from the enzyme E. You discover two inhibitors of this enzyme, I and J. I is a competitive inhibitor of the substrate A, and has nearly double the molecular weight of J. On the other hand, J is a mixed inhibitor of enzyme E, and its inhibitory effect on Km / Vmax (the slope effect from the double reciprocal plot) is greater than that of 1 / Vmax (the intercept effect in a double reciprocal plot). That is Kis < Kii . At low pH, the conversion of EX to EQ is greatly slowed, kcat is decreased, and the intercept effect of inhibitor J is elevated, that is, the value of Kii is diminished. When a high, fixed concentration…