1.The kinetics of an enzyme are measured as a function of substrate concentration in the presence or absence of an inhibitor. The following results are obtained: (nm/mn) Unitsi Km > mm) Substrate Concentration units: Vmas > Velocity (nanomoles/min) No inhibitor in millimoles (mM) With inhibitor 55.8 x 10 54.9 x 10 51 x 10 47 x 10 40 x 10' 28 x 10 18.7 x 10 11.2 x 10 2.7 x 10 55.7 x 10 53.3 x 103 45 x 10 37.3 x 10 28 x 103 16 x 10 9.3 x 10 100 10 1 0.5 0.2 0.1 0.05 Not tested 0.01 Not tested o.2 05 S. 2. A. What are the Vmax and Km values for the enzyme-catalyzed reaction without inhibitor? Please give units and explain your reasoning. (See note.) Vmons > Km B. (i). What are the apparent Vmax and Km Values plus inhibitor? (ii). Is this a case of competitive or noncompetitive inhibition? How did you know?

1.The kinetics of an enzyme are measured as a function of substrate concentration in the presence or absence of an inhibitor. The following results are obtained: (nm/mn) Unitsi Km > mm) Substrate Concentration units: Vmas > Velocity (nanomoles/min) No inhibitor in millimoles (mM) With inhibitor 55.8 x 10 54.9 x 10 51 x 10 47 x 10 40 x 10' 28 x 10 18.7 x 10 11.2 x 10 2.7 x 10 55.7 x 10 53.3 x 103 45 x 10 37.3 x 10 28 x 103 16 x 10 9.3 x 10 100 10 1 0.5 0.2 0.1 0.05 Not tested 0.01 Not tested o.2 05 S. 2. A. What are the Vmax and Km values for the enzyme-catalyzed reaction without inhibitor? Please give units and explain your reasoning. (See note.) Vmons > Km B. (i). What are the apparent Vmax and Km Values plus inhibitor? (ii). Is this a case of competitive or noncompetitive inhibition? How did you know?

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter21: Photosynthesis

Section: Chapter Questions

Problem 15P

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