1.2 In the food industry, food safety is a major concern. For example, the food-dye, FD&C Yellow 6, is approved for use in foods but the acceptable daily intake by children is 3.75 mg/kg due to concerns like hyperactivity. A UV-vis spectroscopy calibration curve for yellow dye 6 (absorbance vs. concentration in mol/L) was prepared at 485nm. The equation of the linear region between 0.2 and 1.4 absorbance is: y = 19896x Determine the molar extinction coefficient of yellow dye 6 at 485 nm. a. Assume the pathlength is 1.000 cm.

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1.2 In the food industry, food safety is a major concern. For example, the food-dye,
FD&C Yellow 6, is approved for use in foods but the acceptable daily intake by children
is 3.75 mg/kg due to concerns like hyperactivity.
A UV-vis spectroscopy calibration curve for yellow dye 6 (absorbance vs. concentration
in mol/L) was prepared at 485nm. The equation of the linear region between 0.2 and 1.4
absorbance is:
y = 19896x
Determine the molar extinction coefficient of yellow dye 6 at 485 nm.
a.
Assume the pathlength is 1.000 cm.
Transcribed Image Text:1.2 In the food industry, food safety is a major concern. For example, the food-dye, FD&C Yellow 6, is approved for use in foods but the acceptable daily intake by children is 3.75 mg/kg due to concerns like hyperactivity. A UV-vis spectroscopy calibration curve for yellow dye 6 (absorbance vs. concentration in mol/L) was prepared at 485nm. The equation of the linear region between 0.2 and 1.4 absorbance is: y = 19896x Determine the molar extinction coefficient of yellow dye 6 at 485 nm. a. Assume the pathlength is 1.000 cm.
b.
You want to use the calibration curve to estimate the concentration of
yellow dye 6 in 2.00 mL samples of lemon-lime sports drink by extrapolation. You
measure the following data:
Sample
Absorbance
1.64
1.55
A
B
1.20
D
1.00
E
0.54
F
0.19
Which samples can you reliably estimate the concentration of yellow dye 6 for, if you
extrapolate from the calibration curve?
) You decide to add one more pair of data points to the calibration curve
using a standard stock solution of 10.00 mL yellow dye 6 that is 7.5 x 10-5 M. You remove
3.00 mL of the stock, add it to a 10.00 mL volumetric flask and make it up to the mark
with de-ionized water. What is the final concentration of the solution?
d.
Based on your answer in c above, is the concentration low enough to
extend the range of the calibration curve? Briefly explain.
Transcribed Image Text:b. You want to use the calibration curve to estimate the concentration of yellow dye 6 in 2.00 mL samples of lemon-lime sports drink by extrapolation. You measure the following data: Sample Absorbance 1.64 1.55 A B 1.20 D 1.00 E 0.54 F 0.19 Which samples can you reliably estimate the concentration of yellow dye 6 for, if you extrapolate from the calibration curve? ) You decide to add one more pair of data points to the calibration curve using a standard stock solution of 10.00 mL yellow dye 6 that is 7.5 x 10-5 M. You remove 3.00 mL of the stock, add it to a 10.00 mL volumetric flask and make it up to the mark with de-ionized water. What is the final concentration of the solution? d. Based on your answer in c above, is the concentration low enough to extend the range of the calibration curve? Briefly explain.
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