10 Wavelength, à. (nm) ɛ (Red, µ.M¯lcm¬ ɛ(Yellow, µ.Mr'cm=l) Absorbance 500 0.0369 0.000549 0.405 430 0.00506 0.0167 0.474 An orange colored solution is prepared by mixing a red dye solution with a yellow dye solution and the absorbance was measured at the analytical wavelengths of 430 nm and 500 nm. The table provides the molar absorptivity for the red and yellow dye at each analytical wavelength. Calculate the concentration (in µM) of the red and yellow dye in the mixture. [red] = u.M [yellow] = uM
10 Wavelength, à. (nm) ɛ (Red, µ.M¯lcm¬ ɛ(Yellow, µ.Mr'cm=l) Absorbance 500 0.0369 0.000549 0.405 430 0.00506 0.0167 0.474 An orange colored solution is prepared by mixing a red dye solution with a yellow dye solution and the absorbance was measured at the analytical wavelengths of 430 nm and 500 nm. The table provides the molar absorptivity for the red and yellow dye at each analytical wavelength. Calculate the concentration (in µM) of the red and yellow dye in the mixture. [red] = u.M [yellow] = uM
Chapter25: Instruments For Optical Spectrometry
Section: Chapter Questions
Problem 25.22QAP
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