The total absorbance of a solution is the sum of the absorbances of all different materials present. AT = Aa + Ab + Ac ... etc. At 427 nm, the molar absorbtivity of Yellow #5 is 2.73×10ª M-1-cm¯1. At 427 nm the molar absorptivity of Red 40 is 7.49x10³ M-1-cm-1. Calculate the total absorbance of a solution in which the concentration of Yellow #5 = 2.00x10-5 M and the concentration of Red 40 = 4.80×10-5 M, when measured at 427 nm in a 1.00 cm cell.

Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter13: An Introduction To Ultraviolet-visible Molecular Absorption Spectrometry
Section: Chapter Questions
Problem 13.24QAP
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4. The total absorbance of a solution is the sum of the absorbances of all different materials present. AT = Aa +
Ap + Ac .. etc. At 427 nm, the molar absorbtivity of Yellow #5 is 2.73×10°M-1-cm1. At 427 nm the molar
absorptivity of Red 40 is 7.49x103 M-1.cm-1. Calculate the total absorbance of a solution in which the
concentration of Yellow #5 = 2.00x10-5 M and the concentration of Red 40 = 4.80x10-5 M, when measured at
427 nm in a 1.00 cm cell.
Transcribed Image Text:4. The total absorbance of a solution is the sum of the absorbances of all different materials present. AT = Aa + Ap + Ac .. etc. At 427 nm, the molar absorbtivity of Yellow #5 is 2.73×10°M-1-cm1. At 427 nm the molar absorptivity of Red 40 is 7.49x103 M-1.cm-1. Calculate the total absorbance of a solution in which the concentration of Yellow #5 = 2.00x10-5 M and the concentration of Red 40 = 4.80x10-5 M, when measured at 427 nm in a 1.00 cm cell.
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