10. The concentration of haemoglobin in a blood sample was determined by spectrophotometry. A standard curve of the absorbance at 412 nm of several solutions of known haemoglobin concentrations was created. The data for the standard curve is shown below. a. Calculate the linearity coefficient (r), y-intercept and slope. b. What is the concentration (in pg/mL) of haemoglobin in your sample if the absorbance obtained at 412 nm was 0.303? Absorbance Concentration of standard solution (pg/ml) (412nm) 0.069 1 0.113 0.201 2 4 0.377 8 0.730 16
10. The concentration of haemoglobin in a blood sample was determined by spectrophotometry. A standard curve of the absorbance at 412 nm of several solutions of known haemoglobin concentrations was created. The data for the standard curve is shown below. a. Calculate the linearity coefficient (r), y-intercept and slope. b. What is the concentration (in pg/mL) of haemoglobin in your sample if the absorbance obtained at 412 nm was 0.303? Absorbance Concentration of standard solution (pg/ml) (412nm) 0.069 1 0.113 0.201 2 4 0.377 8 0.730 16
Chemistry & Chemical Reactivity
10th Edition
ISBN:9781337399074
Author:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Publisher:John C. Kotz, Paul M. Treichel, John Townsend, David Treichel
Chapter4: Stoichiometry: Quantitative Information About Chemical Reactions
Section: Chapter Questions
Problem 77PS
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