1,000 bp- 700 bp. 500 bp 200 bp. 100 bp- Lane 12345678 6 8 Sample Molecular weight ruler Positive control DNA (+) PCR Sample (S) PCR Sample (C) PCR Sample (D1) PCR Sample (D2) PCR Sample (B/D3) Negative control (-) 1 2 3 4 5 6 7 8 I 1 I I
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Band, Band Size, and what bands corresponds?
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- DNA Profiles as Tools for Identification A PCR-based paternity test is conducted using STRs that consistently produce a unique DNA fragment pattern from a single chromosome. Examining the results of the following Southern blot, which male(s) can be excluded as the father of the child? Which male(s) could be the father of the child?You are given a tube containing 275 ng of purified PCR product (DNA) that is 1262 bp long. How many picomoles of PCR product are in the tube? The average molecular weight of a deoxynucleotide monophosphate is 328 g/mol.PCR is a very useful method in biotechnology because it does not require the 6 or more different key proteins/enzymes required for DNA replication in living cells. Explain how and why this technique only requires 1 enzyme to make lots of DNA.
- Polymerase Chain Reaction (PCR) works by exposing the reaction to a series of temperature changes. In 150 words or fewer, describe 1.) the different stages of a single PCR cycle, 2.) how many cycles of PCR your reactions will undergo, and 3.) why multiple cycles are necessary.In a PCR-based crime scene investigation, similar to the one presented in the lab module with Brother Y and Brother X, there is a sample of DNA from a crime scene that is likely to belong to the guilty party. Based on the gel photo below, which shows the results of an electrophoresis gel following PCR amplification at one locus of 5 DNA samples - one crime scene sample and 4 suspects - which suspects can be excluded from this investigation? [Keep in mind that it is not possible for a heterozygous person to leave only one allele at a crime scene. If any one allele does not match, then that suspect is eliminated.] choose all that applyIn lane 1, a size standard was loaded, which contained a mix a DNA fragments known to be 1000 bp, 700 bp, 500 bp, 200 bp, and 100 bp. When the gel was run and stained, the following photograph of the gel was taken. Click directly on the band in lane 1 that represents the DNA fragment that is 500 bp in size.
- What would you need to do to preform a successful PCR but only had thermolabile DNA polymerase? Can be kept relativity simpleAfter you did the amplification by PCR, you run the product on 2% gel. Tell me how did you prepared the gel if the tray is 150 ml? how many milliliters of the TBE and how many grams of the Agarose? Draw a picture (On a paper or on computer) for the gel that you are expecting, the gene that you amplified is approximately 200 bpGel Electrophoresis Background and Protocol: Gel electrophoresis is a laboratory technique that separates molecules by size using an electric current. The test has a positive and negative side. Do you believe DNA should be loaded on the positive (red) side or the negative (black) side? Please explain why using scientific reasoning. Will larger DNA bands be closer or farther away from the well where you administered samples? Why?
- Chemistry Biochemistry: Polymerase Chain Reaction Please compare and contrast these three OR make table: 1.qPCR 2. RT-PCR 3. PCROur PCR samples already contain loading dye, but sometimes this isn’t the case. If your samples didn’t already contain dye and you wanted to load your PCR sample onto an agarose gel, you’d need to add loading dye to the proper concentration. There is a 6X loading dye available for use; how many µl of this loading dye will you add to 10 µl of your sample so that it is at a 1X working concentration? Show your work.If you were to re-do this lab again with the same basic constraints (one DNA source, multiple enzymes), what would you do to make the gel a clear, readable DNA fingerprint? Would you change any restriction enzymes? Would you run the gel longer? Explain.