14. A student makes a smear by applying a bacteria onto the slide. A simple stain is performed, but when the student looks under the microscope, the organisms are misshapen and look weird. What did the student do wrong when making the smear?
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- 12. A student makes a smear by applying a bacteria onto the slide. A simple stain is performed, but when the student looks under the microscope, no organisms are seen. What did the student do wrong when making the smear?3. Draw the specimen observed under endospore staining 4. Draw the specimen observed under positive simple staining13. A student makes a smear by applying a LARGE amount of bacteria onto the slide. A simple stain is performed, but when the student looks under the microscope, individual organisms can’t be distinguished. What did the student do wrong when making the smear?
- 4. You partner stained bacterial cells and saw only the background and not the actual cell was stained. Your partner thought this was a mistake. Please explain what type of staining method this is, how it works and why the background and cell is not stained.The specimen for an electron microscope is always a. stained with dyes c. killed b. sliced into thin sections d. viewed directlyobserve the transparent bacteria under bright field microscope, we use a. Simple staining b. Gram staining c. Negative staining d Direct staining eAll the above
- 13. A staining that divides organisms into two groups depending on their staining is referred to as __________. a) simple staining b) differential stain c) structural staining d) morphological stainingWhy are staining methods used in microbiology? Choose all that apply A. Staining results can have medical implications for an infected B. To clearly see the internal structures of the bacteria C. The stains can be used to dinginuish between bacteria type D. It is easier to very small microscope.Explain how bacterial cells would look in the gram staining procedure if the following mistakes were made: a. Decolorizer left on too long b. Decolorizer not left on long enough c. Slide not heat-fixed before staining
- 1. When performing a bacterial lab, what could happen that could lead to incorrect results (potential pitfalls during the lab?)2. What does a mordant do in the Gram stain procedure? Which reagent in the Gram stain is the mordant?the purpose of a mordant in Gram stain is a. to prevent the crystal violet from leaving the cell b. to make bacteria cell larger c. to fix iodine inside the cell d. to remove simple stain