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- Radiolabeling with 14C-Glutamate Describe the labeling pattern that would result from the introduction into the TCA cycle of glutamate labeled at Cy with 14C.In the context of fatty acid oxidation to acetyl CoA, indicate whether each of the following substances is involved in (1) fatty acid activation, (2) fatty acid trans-port, or (3) b-oxidation pathway. More than one choice may be correct in a given situation. a. AMp b. FAD c. Acyl CoA d. H2OConsider the docosanoic acid, C21H43CO2H a. Label the a and B carbons b. Draw the acyl CoA derived from this fatty acid c. How many acetyl CoA molecules are formed by complete B-oxidation? d. How many cycles of B-oxidation are needed for complete oxidation? e. How many molecules of ATP are formed from the complete catabolism of this fatty acid?
- If the w-carbon atom of a fatty acyl CoA molecule with a C18 chain is labeled with C14, how many rounds of β-oxidation are required to produce a radioactively labeled acetyl CoA? a. four b. five c. six d. seven e. eight f. nine22. Which hormone is NOT derived from cholesterol? a. oxytocin b. testosterone c. both a and b d. neither a nor b 23. Which lipoprotein mostly contains triacyglycerol (TAGs)? a. HDL b. VLDL c. LDL d. chylomicrons 24. Which enzyme in cholesterol synthesis is inhibited by statins? a. HMG-CoA synthase b. HMG-CoA reductase c. both a and b d. neither a nor b 25. Which lipoprotein prevents the formation of plaque in the artery? a. HDL b. VLDL c. LDL d. chylomicrons 26. In which test/s will triacylglycerol yield a positive result? a. acrolein b. translucent spot c. both a and b d. neither a nor b 27. In which solvent will corn oil be soluble? a. hexane b. benzene c. both a and b d. neither a nor b 28. In which test/s will cholesterol yield a positive result? a. acrolein b. Huble's test c. both a and b d. neither a nor b 29. Which sample would test negative in saponification test? a. cholesterol b. 18:2 fatty acid c. both a and b d. neither a nor b 30. What test could distinguish…CHOOSE THE CORRECT LETTER 1.What are the B-oxidation products of lauric acid (C12H2402), a major component of coconut oil?A.6 acetyl-CoA, 6 NADH, 6 FADH2B. 6 acetyl-CoA, 5 NADH, 5 FADH2C. 5 acetyl-CoA, 6 NADH, 6 FADH2D. 5 acetyl-CoA, 5 NADH, 5 FADH2 2.What is the mechanism of ATP synthesis in glycolysis?A. substrate level phosphorylationB. reductionC. oxidationD. oxidative phosphorylation 3.Which of the following enzymes need ATP as a substrate in the reaction?A.phosphoglycerate kinaseB. phosphofructokinaseC. pyruvate kinaseD.glyceraldehyde-3-phosphate dehydrogenase
- Order the steps that results in a triacylglycerol containing two palmitic acids and one stearic acid. a. Diacylglycerol reacts with an fatty-acyl-CoA b. Phosphate is remove from phosphatidic acid to form diacylglycerol c. Two of the fatty acyl-CoAs react with glycerol-3-phosphate d. An elongase produce stearic acid e. Stearoyl-CoA is synthesized…The reaction of acetyl-CoA and oxaloacetate in the TCA cycle forms a. 5-carbon α-ketoglutarate b. 4-carbon Succinyl-CoA c. 6-carbon citrate d. 6-carbon α-ketoglutarate e. 4-carbon citrateStarting with acetyl-S-enzyme-1 and malonyl-CoA, how many molecules of acetyl- CoA are needed to synthesize an 18-carbon fatty acid (C18:0)? How many molecules of CO2 are released in this process?
- If the w-carbon atom of a fatty acyl CoA molecule with a C12 chain is labeled with C14, how manyrounds of β-oxidation are required to produce a radioactively labeled acetyl CoA? one two four five How many molecules of glucose are required to provide the carbon for the synthesis of onemolecule of tripalmitoylglycerol? 4.5 8.5 10.5 12.5 Need more informationMany components of the glycolytic pathway and the citricacid cycle are direct exit or entry points to metabolic pathways ofother substances. Indicate another pathway available to the following compounds:(a) Fructose-6-phosphate(b) Oxaloacetate(c) Glucose-6-phosphate(d) Acetyl-CoA(e) Glyceraldehyde-3-phosphate(f) -Ketoglutarate(g) Dihydroxyacetone phosphate(h) Succinyl-CoA(i) 3-Phosphoglycerate(j) Fumarate(k) Phosphoenolpyruvate(l) Citrate(m) PyruvateMalonyl-CoA synthesized by the action of acetyl-CoA carboxylase II is primarily used:a. To synthesize fatty acidsb. To inhibit fatty acid oxidationc. Both a and bd. Neither a nor b
