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- Table 2. Volume of BSA, protein content, and absorbance readings of reference solutions Solution Volume of BSA standard solution (μL) Protein content(μg/mL) Absorbance value At 595 nm 1 0 0 0 2 10 1 0.022 3 30 3 0.065 4 50 5 0.106 5 70 7 0.178 6 100 10 0.299 7 120 12 0.380 Make a graph by plotting the absorbance values versus the BSA protein content (in μg) for theseven reference solutions. When constructing the graph, be…Calculate the pH of the solution that results following addition of 10 mL of 1 M NaOH to 40 mL of 1 M NH4ClAn aqueous solution contains 0.448 M dimethylamine ((CH3)2NH).How many mL of 0.240 M hydroiodic acid would have to be added to 150 mL of this solution in order to prepare a buffer with a pH of 10.500? ______________ml