2 ATP + HCO₂ + NH₂ 140 CPS-1 H₂N OPO,2 H₂N 2 ADP, P CH₂ HỘ NHỎ coo 1 0=UN NH₂ P OTC ARG1 H₂O NH₂ H₂H Coo NH CHI CH₂ Coo CH₂ 7 CH₂ HỘ NHĨ coo 4 5 Which of the following is a carbon input to this pathway? (Numbers refer to the above key) 1 2 3 4 None of these is a carbon input NH CH₂ CH₂ CH₂ HỒ NH coo ASL -8---8 CH₂ HỘ NHĨ coo CH ASS ATP PP L-Asp AMP 1 L-ornithine 2 carbamoyl phosphate 3 L-citrulline 4 argininosuccinate 5 fumarate 6 L-arginine 7 urea L-Asp L-aspartate CPS-1 carbamoyl phosphate synthetase I OTC Ornithine transcarbamoylase ASS argininosuccinate synthetase ASL argininosuccinate lyase ARG1 arginase 1 OH OH NH₂
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- One process catalyzed by NADHNADH dehydrogenase is NADH+H^++ubiquinone ↽−−⇀ NAD+ubiquinolNADH+H^++ubiquinone ↽−−⇀ NAD^++ubiquinol The standard reduction potentials for the half‑reactions are given in the table. Oxidant Reductant ?′0 ubiquinone+2H++2e−ubiquinone+2H++2e^− ubiquinolubiquinol 0.045 NAD^++H^++2e−NAD^++H^++2e^− NADHNADH –0.32 Calculate Δ?′0 for the reaction as shown. Δ?′0=____(V) Calculate Δ?′0 . Δ?′0=____(kJ/mol)Under standard conditions, is the oxidation of ubiquinol (Coenzyme Q) by O2 sufficiently exergonic to drive the synthesis of ATP? If yes, how many ATP can be synthesized assuming 100% efficiency?Consider the coupled reactions: 1,3-BPG → 3-PG + Pi ΔG°'= -16.3 kcalmolkcalmol ATP → ADP + Pi ΔG°'= -7.3 kcalmolkcalmol Which of the following is TRUE about the total ΔG°’ for the coupled reactions? -9.0 kcal/mol; spontaneous +9.0 kcal/mol; nonspontaneous -23.6 kcal/mol; spontaneous +23.6 kcal/mol; nonspontaneous
- Below are the reduction reactions for oxygen and FAD. ½202 + 2e + 2H+ → H20 E°' = 0.83 V FAD + 2e + 2H* → FADH2 E" = -0.22 V What is the potential (E") for the oxidation of FADH by oxygen? What is the AG®' for the oxidation of FADH, by oxygen? If we assume that the pumping of protons in conjunction with the oxidation of FADH, requires 120 kJ, what percentage of the energy from FADH oxidation is stored in the proton gradient (think about how many protons are pumped when FADH, transfers electrons to oxygen)?a newly identified bacterium called Nomore biochem is unable to synthesize ubiquinone. A mobile electron carrier called CXC3 is used as a substitute. From the information provided in the table, calculate delta G' and Keq value at 298K for the redox reaction that occurs in the Nomore Biochem electron transport chain. (constants: R=8.3 J/degree x mol, F= 96.1 kJ/v x mol Half Reactions E' (V) ubiquinone + 2e- + 2H+--> Ubiquinol + H2 0.045 NAD+ + 2e- + 2H+ --> NADH + H+ -0.320 CXC3 + 2e- + 2H+ --> CXC3H2 -0.450 explain the impact that using CXC3 instead of ubiquinone will have on ATP production in the cell. How might the cell adapt to this situation?For the Complex III in the electron transport chain: Complex III step 1: UQH2 is oxidized in a 2 electron process. Cytochrome c is reduced and UQ is reduced to UQH in two 1 electron processes. Complex III step 2: UQH2 is oxidized in a 2 electron process. Cytochrome c is reduced and UQH is reduced to UQH2 in two 1 electron processes. The necessary standard reduction potentials are: UQ + 2H+ + 2e- UQH2 E° = 0.06 V cyt c (Fe3+) + e- cyt c (Fe2+) E° = 0.254 V UQ + H+ + e- UQH. E° = 0.03 V UQH. + H+ + e- UQH2 E° = 0.19 V Calculate the total redox potential of the complex. Now calculate how many moles of protons can be translocated across the inner mitochondrial membrane if translocation of 1 mole requires 23 kJ. Calculate the free energy available for proton translocation assuming a 2electron process for each complex.
- Consider the coupled reactions: 1,3-BPG → 3-PG + Pi ΔG°'= -16.3 kcalmolkcalmol ATP → ADP + Pi ΔG°'= -7.3 kcalmolkcalmol Which of the following is TRUE about the total ΔG°’ for the coupled reactions? A. +23.6 kcal/mol; nonspontaneous B. -23.6 kcal/mol; spontaneous C. +9.0 kcal/mol; nonspontaneous D. -9.0 kcal/mol; spontaneousIf a reaction has a ΔG°′ value of at least −30.5 kJ · mol−1, suffi -cient to drive the synthesis of ATP (ΔG°′ = 30.5 kJ · mol−1), can it still drive the synthesis of ATP in vivo when its ΔG is only −10 kJ · mol−1? Explain.The complete oxidation of palmitoyl-CoA to carbon dioxide and water is presented by the overall equation: Palmitoyl-CoA + 23O2 + 108Pi + 108 ATP + 23H2O → CoA + 16 CO2 + 108 ATP + 23H2O Water is also produced in the reaction ADP + Pi → ATP + H2O But not included as a product in the overall equation. Why?
- If the Go for ATP hydrolysis into ADP + inorganic phosphate is 7.3 kcal/mole, and the Go for glutamine synthesis from glutamic acid and NH3 is +3.4 kcal/mole, calculate the average Go for coupling these two reactions (glutamic acid + NH3 + ATP glutamine + ADP + inorganic phosphateA total of 30.5 kJ mol-1 of free energy is needed to synthesise ATP from ADP and Pi when the reactants and products are at 1.0 M concentrations and the temperature is 25oC. Because the actual physiological concentrations of ATP, ADP, and Pi are not 1.0 M, and the temperature is 37oC, the free energy required to synthesise ATP under physiological conditions is actually ~ 46.2 kJ mol-1. A 68 kg adult requires an energy intake of 8,550 kJ of food per day (24 hours). Calculate the mass (in Kg) of ATP synthesised by a human adult in 24 hours, assuming that the percentage efficiency of converting inputted calories in ATP is 50%. What percentage of the body weight does this represent?Consider the following equilibrium at 25ºC :Glucose-1-Phosphate Glucose-6-PhophateUsing the equilibrium concentrations of [Glucose-1-Phosphate] = 0.35 M and [Glucose-6-Phosphate] = 1.65 M, calculate BOTH K′eqand Gº′ for this reaction. Is this reaction exergonicor endergonic? R = 8.314 J/K·mol