add 2.75 mL out of 5.0 mL of an undiluted sample to 125.25 mL. ad, you add all 5.0 mL to the 125.25 mL. What was the intended the actual dilution?
Q: You are preparing to give your patient 3mg of Ondansetron. Ondansetron comes packaged as 2mg/mL. How…
A: Ondansetron is an anti-emetic medication prescribed for nausea and vomiting. It belongs to a class…
Q: You want to set up a 1:10 dilution series, so that you can plate a 10-1, 10-2, and a 10-3dilution;…
A: It is basically done for counting the microorganisms like viruses, bacteria etc from an unknown…
Q: 0.0050 M KOH PH VALUE
A: KOH stands for potassium hydroxide. It is an inorganic compound with the chemical formula of KOH.
Q: to dilute a sample by 1/3. For a final volume of 27 ml, how much sample would you add to how much…
A: The concentration of the solution fluctuates as it is diluted, but the number of moles of reactant…
Q: You were instructed to add 0.2 ml of kombucha to 9.8 ml of fresh tea, but instead added 2.0 ml of…
A: Dilution is defined as the ratio of stock volume to the final volume. Here, final volume is the sum…
Q: Appropriate pippetor for measuring 10ul of liquid is: 200 1000 20
A: INTRODUCTION Pipette Pipette is a laboratory equipment used to transfer small quantities of liquid.
Q: Report how to make a 250.mL of 50pM sodium acetate and 0.01u EDTA using solid sodium acetate and…
A: Molarity (M) indicates the number of moles of substance per liter of solution (moles/Liter) and is…
Q: Calculate the CFU present in 150 mL water sample and b) dilution factor up to 5. Consider the…
A: The serial dilution process is used to calculate the number of microorganisms present in a…
Q: Is the time required to Elute substance A, greater if N=1000 or N=2000?
A: N is number of theoretical plates in column Retention time (RT) is a measure of the time taken for a…
Q: When 11 milliliters are transferred from his sample to a dilution vial containing 99 ml, what is the…
A: Given Volume of stock transferred = 11ml Vial volume = 99ml Both are same unit so don't have to…
Q: How many CFU per ml are there in the original sample using the following dilution and plating…
A: "Microbiology" is a hugely important field in both basic and applied research. New and fascinating…
Q: relative density of the insoluble powder
A:
Q: You record the absorbance of your unknown BSA sample at 0.276. What is the concentration in ug/mL of…
A: Standard curve can be used to determine the concentration of unknown sample by determining their…
Q: 1. Choose the most appropriate glassware (1.0 mL pipette, 50 mL Erlenmeyer flask, 100.0 mL graduated…
A: For measuring any quantity of a substance, the appropriate glassware is chosen according to the…
Q: BSA mg/ml O.D. 550nm 1 0.12 2 0.23 3 0.34 4 0.41 5 0.52 Use the values above to draw a graph
A: BSA Bovine serum albumin is a albumin protein which is extracted from cow and used in experimental…
Q: What volume of 10X TBE buffer should you use to make 100mL of 1X TBE buffer? 10mL 1mL 0.1mL 100mL
A: TBE buffer stands for Tris-Borate-EDTA buffer. It is used for both agarose and polyacrylamide gel…
Q: How would you perform a 100-fold dilution, if you want the final volume to be 15mL? What volume of…
A: Dilution is the process of decreasing the concentration of a solute in a solution. To dilute a…
Q: what will be the possible results in testing the solubility of oil in the water?
A: Polar or hydrophilic molecules are soluble in water and non-polar or hydrophobic molecules are…
Q: A solution measuring pH 12 is _________ times __________ concentrated with H+ than a solution…
A: The term "pH" was first described by Danish biochemist Soren Peter Lauritz Sorensen. pH is defined…
Q: Aisha wants to dissolve some salt in 25 cm³ of water. Which equipment is most suitable for measuring…
A: Different measuring equipment is used in the laboratory to measure liquids. Identifying the proper…
Q: Using 25 µL of a patient's serum, how much diluent would be needed to create a 1:2 dilution? ul
A: Firstly, we convert the 25ul into ml . i.e. 25 ul = 0.03 ml Then, there will be the multiplication…
Q: How many grams of NaOH would you need to make 3,982 mL of a 3 M NAOH solution (m.w. = 40.00 g/mol)?…
A: By the concept of molarity, we can determine the concentration of a solution. It is the total number…
Q: Convert 12 pints to an equivalent volume in quarts.
A: In USA there's a dry pint and a liquid pint which measure different.Dry pint measures 550.6 ml and a…
Q: 8.5gm/250ml = (x)gm/630ml
A: The amount of chemical dissolved in the solvent = 8.5gm The volume of the solvent…
Q: Which of the following reagents will you include in the blank (negative control) for this…
A: A variable is any quantity we can measure. In any experiment there are three variables:…
Q: | || | | || | || 1 45 Using the figure, which lane has a poor quality sample and is possibly…
A: Electrophoresis (Gel electrophoresis) is commonly used in labs to study a mixture of biomolecules…
Q: 100 mL of 2.5 X NaOH solution from 50X NaOH solution
A: Given Values: Stock solution concentration (NaOH) = 50 X Final Solution concentration (NaOH) = 2.5 X…
Q: 28. 29. 30. -30 -75 -100 65 -90 10 55 80 Graduated cylinders #28-30 were mL. measuring increments of…
A: A graded cylinder's scale divisions are usually determined by its size. The 50-mL graduated…
Q: To determine the cfu/ml of a pondwater sample, you perform 1/10, 1/100, 1/1000 and 1/10,000 serial…
A: Serial dilution is a technique which is adopted to dilute the highly concentrated microbial culture…
Q: Why is there a need to use acids and boil the sample in maceration?
A: Maceration is a common method of obtaining medicinal plants, it is an extraction process in which…
Q: You have a 6 M solution of stock A. You do the following serial dilutions: Add 1 ml of stock A to…
A: Dilution is calculated by stock volume to the final volume. Solution = Stock volume/ (stock volume+…
Q: measurements using the pH meter and the pH paper? Compare the initial pH and the final pH of each…
A: pH paper is a filter paper that has a natural soluble dye that is used to test the acid or alkaline…
Q: What is the volume (in mL) of ethanol in the bottle?
A: Volume is calculated by the % by volume formula, that is volume of solute dissolved in the volume of…
Q: Finally, draw the standard curve for BSA. Reagents Blank Tube Tube Tube 2 3 Tube Tube Tube Tube 5 4…
A: BSA is the bovine serum albumin that is a protein concentration used as a reference in the protein…
Q: A 25.00 mL sample of 0.320 M LİÖH is titrated with 0.750 M HNO3 at 25 °C. Calculate the initial pH…
A: Titration is a quantitative chemical analysis to determine the concentration of an identified…
Q: U, was used. Write the ionization euation for thin
A: weak acid is an acid that partially dissociates into its ions in an aqueous solution or water. In…
Q: Determine the serial dilution used from the original water sample (100 mL) with 4.8 x 105 CFU as…
A: Introduction: Dilution is the process of reducing the concentration of a solute in a solution by…
Q: Complete the Table Volume capacity and precision a graduated cylinder 50 mL; P=0.1 mL Volume…
A: Diluting a concentration solution: In order to prepare a lower concentration solution from a given…
Q: You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) 0 1 2 3 4 5…
A: Even when the concentration of BSA (Bovine Serum Albumin) is 0 , an absorbance of 0.158 was…
Q: An injectabie contains 5 mg of active ingredient in each 10-ml ampule. If the dose of the drug or a…
A: Drug administration is one of the major job responsibilities of a clinical nurse. Drugs are the…
Q: How much liquid media should you put in your dilution blank if you wanted to serially dilute a1 ml…
A: Serial dilution of the original sample containing microorganisms to achieve countable colonies on…
Q: You are observing the results from a standard plate count and you observe 53 CFUS. To make the plate…
A: The correct answer is option (4) 5.3 x 105 CFU/ml CFU/ml stands for colony-forming units per…
Q: 9% dextrous solution (9 mg per 100 ml. of solution) is given
A: A. 1a. In order to calculate flow rate of dextrose we have to use following formula 2.18 L = 2180…
Q: Report how to make a 250.mL of 50pM sodium acetate and 0.01p EDTA using solid sodium acetate and…
A: Given Values: The concentration of sodium acetate in the solution = 50 μM The volume of the solution…
Q: What is the role of indicator in determining if the substance is acid or base? (Note that the…
A: pH indicators are compounds which are natural dye. Those indicate if the solution is acidic or basic…
Q: Using the table above, make the following unit conversions. Determine th point the appropriate…
A: 1. 25/1000 = 0.025 2. 3*1000000= 3000000 3. 150/10 = 15 4. 5000/1000 = 5 5. 0.075 kl
Q: What does “1 Millimolar” (1mM) mean
A: Milli is a suffix in measurement meaning 1/1000 or 10^-3.
Q: Peppermint Spirit, NF, BP (Solution with maceration) Formula Peppermint oil Fresh peppermint leaves…
A: Peppermint spirit is used for treating stomach and intestines problems. It is indicated for…
Q: Desired Desired Initial Volume of Stock or Volume of Concentration Volume Concentration Previous…
A: In the question, we are given a table in which we know the desired concentration, desired volume and…
Trending now
This is a popular solution!
Step by step
Solved in 3 steps
- 3.1 You can perform a specific staining method to confirm the purity on the culture. Describe this staining method. 3.2 Describe the results obtained from 3.1 and comment about the purity of the culture. 3.3 What staining method would you use to verify that the isolate is not producing survivalstructures against unfavourable conditions? Describe the procedure as well.You were instructed to add 1.0ml out of 4.0ml of an undiluted sample to 99ml of sterile diligent you add the entire 4.0ml to 99 ml. a) what was your intended dilution factor? b) what was your actual dilution factor?one lm of milk was added to 9 ml of sterile saline. 0.1ml of the diluted sample was put into a petri dish with melted agar. How many cfu/ml are in the milk if 200 colonies were counted?
- 2.1The number of bacteria in the samples need to be quantified. The laboratory uses a specificprotocol where the dilution factor on the spread plates must be:10-6; 10-7; 10-8Describe how you would prepare the required dilution series using the least amount of dilutionwater. 2.2 Calculate the amount of bacteria in the sample from the following results (show the steps in the calculation): Dilution factor Count10-6 27110-7 3510-8 21At the station there is a 2 mL microcentrifuge tube containing sheep blood and 3 tubes containing the following solutions: 0 mM, 300 mM, 600 mM of sucrose. The tubes are randomly labelled A, B, C. 2 mL of each solution were transferred to a different microcentrifuge tube, 20 ul of blood were then added to each tube, and they were mixed well by inverting the tube for multiple times. They were observed and it was determined whether the solution in each tube was hypotonic (Yes or No) relative to the blood cells. In addition, the solutions were diluted with water to help you determine which solution is which sucrose concentration. What sucrose concentration corresponds with each tube? Tube Hypotonic (Yes/No) Hypotonic after 1:1 dilution with water (Yes/No) Most likely Sucrose concentration (mM) A No Yes B Yes Yes C No No1 ml of a patient sample was added to 999 ml of media. From this 0.1 ml was transferred to the plate. After 24 hours there were 60 colonies on the plate. What is the dilution factor here. What's the number of bacteria per ml of the original patient sample. a. The dilution factor is 1000. The patient sample contains 600 cells/ml b. The dilution factor is 1000. The patient sample contains 600,000 cells/ml c. The dilution factor is 100. The patient sample contains 600,000 cells/ml d. The dilution factor is 10. The patient sample contains 6000 cells/ml e. It cant be determined its too many cells
- In this experiment, a culture was serially diluted to the concentrations below. Each plate was plated with 0.25mL of the dilution. Using the most diluted plate, what is the correct concentration of the original culture? A) 2.0 X 10^-3 cells/mL B) 2.0 X 10^5 cells/mL C) 4.0 X 10^5 cells/mL D) 5.0 X 10^4 cells/mLA plate has 72 colonies with a total dilution factor of 10-7, 0.1 ml was pipetted onto the plate, what was the original CFU/ml concentration of that sample?You are testing unpasteurized milk for the presence of bacterial contamination. Starting from the undiluted milk, you do serial dilutions, and plate 0.5 ml of 4th dilution on agar. If you counted 78 CFUs from the 4th plate, how many bacteria/ml do you expect? (show solution) Do you think standard plate counts are very accurate? Why or why not?
- a pure bacterial culture was diluted by adding a 0.2 mL aliquot to 0.9mL water. Then 0.1 mL of this dilution was plated out, yielding 82 colonies. Calculate the CFU/mL in the original culture.50 g of food is homogenized in 450 mL of water. From this, 1 mL is added to 29 mL of water, and then a 1:90 dilution is made from this, and then 1 mL from this is added to 99 mL of water, and then 100 uL of this last dilution is plated and after incubation 164 colonies are counted.a) What was the overall dilution factor?b) What was the total CFU/g of the original sample?A sample of Earth Alive Soil Activator was diluted by transferring 1 gram into a 9 ml dilution blank (tube A) and then serially diluted five (5) more times by transferring 1 ml of a previous dilution into a tube of 9 ml of sterile water. After making these serial dilutions, 2 ml samples were taken from tubes D, E, and F, added into empty petri dishes, and molten PCA was poured into each petri dish. Tubes A, B, and C were heated in a 80˚C water bath for 30 minutes, 2 ml samples of these tubes were added into empty petri dishes, and molten PCA was poured into each petri dish. These plates were allowed to solidify, and they were incubated at 35˚C for 48 hours. After the incubation period, you counted CFUs on all 6 plates. The results of your CFU counts are contained in table 1. Table 1. CFU counts for 3 plates from the heated dilutions (A-C) and the 3 plates from the non-heated dilutions (D-F) # CFUs Not heated Heated A -- 400 B -- 45 C -- 6 D 350 -- E 31 -- F 2 --…