2:36 PM l TEW What is the unique property of Taq DNA polymerase to use in PCR? b.

Q: Amplified target regions of four different samples were separated using gel electrophoresis.  DNA…

A: Gel electrophoresis is a technique used to separate DNA fragments according to their size. DNA…

Q: DNA polymerases used in PCR _______.

A: The polymerase chain reaction (PCR) is a technique in which millions of copies of the desired DNA…

Q: DNa Mapping using Restriction enzymes lab: We will be aliquoting and delivering 5 μl of enzyme to…

A: A restriction enzyme, also known as restriction endonuclease is an enzyme that cleaves DNA into…

Q: 1. a) A technologist has a 20µl sample of DNA and adds 5µl of loading dye before adding the total…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: 18. What does "highly variable" mean? 19. Why are highly variable regions important in this lab? 20.…

A: Polymerase Chain Reaction ( PCR )-- PCR was first developed in year 1983 by Kary Mullis .Polymerase…

Q: ESTION 12 O words or fewer, explain why we think that RNA, and not DNA, was the hereditary material…

A: RNA and DNA are nucleic acids. RNA is a single strand molecule and DNA is a double stranded…

Q: 5.Statement 1: Restriction enzymes are sequence-specific, which means that they only recognize a…

A: Restriction enzymes also known as restriction endonucleases are the DNA cutting enzymes that are…

Q: 1.a. Match the plasmid illustration to its gel lane. 1.b What is the size of the longest DNA…

A: The gel electrophoresis is the process that is routinely used in laboratories two separate different…

Q: Hyrolysis of DNA Test Results 1. Inorganic Phosphate 2. Purines 3. Deoxyribose

A: Hydrolysis of DNA will cause the DNA backbone to break down and ultimately give the deoxyribose…

Q: What allows the peaks to be different colors?

A: DNA sequencing refers to the determination of the nucleotide sequence in the DNA molecule. Automated…

Q: DNA hybridization process using probe

A: DNA Hybridization is the process of combining two complementary DNA single strands allowing them to…

Q: 1 Restriction enzymes cut DNA molecules atspecific locations to produce restriction fragments…

A: Restriction enzymes refer to the enzymes that generate fragments of DNA (deoxyribonucleic acid)…

Q: 18. An instructor had her students perform this laboratory beginning with setting up their own…

A: Agarose gel electrophoresis is the technique for the separation of DNA fragments on the basis of…

Q: What are the importance of accurate wavelength measurement in purified DNA sample?

A: DNA is a double helical hereditary molecule. The nucleosome is a monomer of the DNA.  In DNA, four…

Q: Describe and contrast the common steps of DNA replication in vivo and the PCR reaction in vitro? 2.…

A: DNA replication occurs at the interphase of the cell cycle. Watson and Crick realized that a single…

Q: Question 1 During nucleic acid hybridization, the probe is labelled for DNA stability to increase…

A: Hybridization permits the distinguishing and cloning of explicit qualities, investigation of levels…

Q: DNA fragments can be separated by: O gel electrophoresis O autoradiography O x-ray crystallography O…

A: Phosphodiester linkages connect DNA strands, which are polymers of deoxynucleoside monophosphates.

Q: If the purity of DNA sample is below 1.8 A260/A280, where did the protein contamination come from?

A: The absorbance ratio at 260 nm & 280 nm is used to determine the quality of DNA & RNA.  A…

Q: Calculate the annealing temperature (Tm in °C) for the PCR primer below. Do not include units when…

A: Answer. Polymerase chain reaction (PCR), the annealing temperature is the temperature during which…

Q: Primer role in PCR

A: Two primers are utilized, one for each of the complementary single strands of DNA released during…

Q: What IS THE difference between Sanger sequencing and Illumina sequencing?

A: Sanger sequencing, also known as the “chain termination method”, is a method for determining the…

Q: Need to understand how to do this: create the complementary strand of DNA to the DNA strand provided…

A: DNA is a double helical structure that comprises of tel strand running in opposite directions. Both…

Q: Explain the importance of adding TE Buffer as the final solution to isolate the concentrated DNA.

A: DNA isolation is a process of extracting nuclear material from a tissue sample. The tissue sample is…

Q: 3. (4 points) The DNA polymerase used in PCR is from a special archaebacteria called Thermus…

A: Taq DNA Polymerase is a highly thermostable recombinant DNA polymerase. It is named after Thermus…

Q: RESULT PROCEDURE A. Salt gargle 1. Dissolves cell membranes B. Filter your extract 2. Breaks open…

A: DNA EXTRACTION PROCESS:- It is the process of extracting the DNA from the rest of the other…

Q: Why was there a greater A260 absorbance reading for your DNA sample that was incubated at higher…

A: Hyperchromicity is the increase in optical density of a substance at a particular wavelength.

Q: How is DNA fragmentation test done?

A: Deoxyribonucleic acid (DNA) is the main constituent of the chromosome. It contains all information…

Q: a) what si the nucleotide at the 5 prime end of the picture?   b) what si the DNA sequence from 5…

A: This is the picture of sanger sequencing gel. The first band is the largest one and subsequent bands…

Q: 25.84 (Chemical Connections 25C) Why is it easier to exclude someone via DNA fingerprinting than it…

A: There are several techniques of biotechnology that are used in the forensic sciences. These…

Q: What is clinical exome test?

A: The exome is the part of the genome which contain all the protein coding sequences.

Q: used to confirm presence of 0.9 kb . dna sequencing  Pcr microarray a and b

A: A microarray is a laboratory tool for simultaneously detecting the expression of thousands of genes.…

Q: Is the DNA soluble in the aqueous solution or alcohol?

A: Solubility of the substance depends on molecular polarity. Polar molecules are hydrophilic…

Q: DNA polymerases used in PCR

A: The polymerase chain reaction (PCR) is a method for rapidly producing millions of copies of the…

Q: naterial used in a PCR reaction

A: Polymerase chain reaction (PCR) may be a methodology wide accustomed quickly create millions to…

Q: How many DNA molecules would exist after 5 PCR cycles?

A: PCR or polymerase chain reaction is an in vitro DNA amplifying technique. It is a very popular…

Q: Results obtained from a Nano Spec. DNA samples collected: ng/uL A260/A80 A260/A230 E.coli genomic…

A: Beer-Lambert Law is applied in absorption spectrometry for biochemical analysis of various…

Q: What is the enzyme that eventually joins the sugar-phosphate backbones of the Okazaki fragments and…

A: DNA recombination technology is a technique in which foreign DNA is joined with host DNA to form…

Q: 79. PCR is a useful tool for early diagnosis of an infectious disease. Comment.

A: Introduction From the above question we will comment on PCR which is useful tool for the early…

Q: What technique was used to photograph DNA in franklins famous photo #51? A- gamma ray photography B-…

A: Imaging with gamma rays is used in nuclear medicine, as well as in court medicine. This technique…

Q: 14. Why denaturation stage is important in PCR? To answer the question, please explain: 11.…

A: The laboratory method that is used for the formation of multiple copies of DNA is quick succession…

Q: xplain how the spin columns were effective in collecting the isolated RNA from the sample and why…

A: The use of spin columns for RNA isolation is a common practice in molecular biology laboratories.…

Q: What is the purpose of restriction enzyme? 2. What is the purpose of the probe in DNA finger…

A: Since you have posted a question with multi- sub parts , we will solve first three sub-parts for…

Q: QUESTION 4: Should PCR primers be complementary to each other?

A: Answer 4-:PCR primers should be complementary to the strands of DNA rather than complementary to…

Q: . Draw the melting curve for primer annealing, labeling the axes. Label the position of the…

A:

Q: What is the significance of using thermostable DNA polymerase for Polymerase Chain Reaction?

A: Polymerase chain reaction (PCR) is a technique for producing multiple copies of DNA in in-vitro…

Q: Figure 4 shows the results from Nanodrop quantification of DNA sample. Sarmple ID E Col (K-12)…

A: DNA is the genetic material in all the living organisms.

Q: 45.) What is the complementary strand for the DNA sequence 5'- CTAAAG-3'? B) 5'- CTTTAG-3' A) 5'-…

A: Complementary deoxyribonucleic acid is a kind of DNA in which the constituent molecules on one…

Q: Why do DNA polymerases fromthermophilic microbes significantly improve the PCRprocedure?

A: Step 1 :Introduction PCR has been used widely in fields such as forensics, genetic testing, and…