RESULT PROCEDURE A. Salt gargle 1. Dissolves cell membranes B. Filter your extract 2. Breaks open the cells 3. Precipitates the DNA (clump the DNA together) C. Mix in a soap solution 4. Separates cell membranes from proteins, carbohydrates and D. Layer cold alcohol over the extract DNA
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- 1. Why is it helpful that the transmission compound light microscopes we studied in lab are both parfocal and parcentered? O Why would it be harder to find a specimen on a slide starting with the 40x objective lens compared to the 4x objective lens? Support your answer with facts about the field of view and the comparison of both objective lenses. If I wanted to observe something, let's a virus that was 50 nm in size which microscope would you recommend me using? Support your answer with facts about the microscope's utility.A student has a compound microscope equipped with 10X ocular lenses and 4X, 10X, 40X and 100X objective lenses. The student has a slide with cells that are -100 microns (1 micron = 10 mm) in diameter, and wants to viewa magnified image of a cell so that the cell appears to be 10 mm in diameter. Which objective lens should the student use? 10X O 40X O 100X 0 4XSomeone has left a tube of unlabeled (poor lab practice) cells on your lab bench. Using differential staining, how would you determine if the cells were from E. coli, beans, or ants? Your hypothesis is that cells are from ants. What stain would you use to differentiate between E. coli and ant cells? Explain and include your expected results.
- This is integral to obtaining the experimental data that lead to the development of this model xray crystallography electrodes electron microscope O patch clampA student has a compound microscope equipped with 10X ocular lenses and 4X, 10X, 40X and 100X objective lenses. The student has a slide with cells that are -100 microns (1 micron = 103 mm) in diameter, and wants to view a magnified image of a %3D cell so that the cell appears to be 10 mm in diameter. Which objective lens should the student use? O 10X O 40X O 100X O 4X Question 7 2 ptsScanning electron microscope can be used for () A. Observe images of different sections of cells B. Observe living cells C. Quantitative analysis of chemical composition in cells D. Observation of cell surface stereomorphology E. Observation of cellular activity
- We will perform a gram stain on our samples, so be sure to bring your instructions on gram staining. Will this procedure stain all cells? A. Yes, all prokaryotic and eukaryotic cells will be stained purple or pink. B. All prokaryotic cells will be stained. Eukaryotic cells will not be stained. C.Mycobacteria and a few other groups with special cell wall structures will not be stained. All other prokaryotic cells will be stained. Eukaryotic cells will be stained pink. D. Mycobacteria and a few other groups with special cell wall structures will not be stained. All other prokaryotic cells will be stained. Eukaryotic cells will be stained purple.O c. bends the light toward the eye and makes the specimen app d. Magnify the apparent size of the specimen Clear my choice What is the purpose of a wet mount slide preparation? Select one: a. None of the answers are correct b. improves the image quality and supports the specimen c. helps to identify diff ent types of cells O d. helps to dilute the specimen. What is the purpose of xylene (xylol) in the isolation and identificat Select one: a. it is used as a clearing agent. O b. it is used as a counter stain. O c. it is used as a decolorizer. d. it is used as staining agent. Clear my choice MacHow to write the methodology? I have a practical of centrifugation, I need to do a lab report, but I don't know how to write the methodology. Here is the introduction: The centrifuge is a common and widely used instrument in the laboratory. In biology,centrifugation is a technique used to separate biological particles or macromoleculesfor instance cell debris, sub-cellular components, proteins, nucleic acids, viralvaccines, antibodies and etc. Principally, the separation is based on the particle size,shape, and density where large and high-density particles travel at a faster rate andwill be sedimented first, followed by other particles. There are many different types ofcentrifuges that are commercially available such as large-capacity low-speedpreparative centrifuges, refrigerated high-speed preparative centrifuges, analyticalultracentrifuges, preparative ultracentrifuges, large-scale clinical centrifuges, andsmall-scale laboratory microfuges.
- Liver tissue was homogenized followed by differential ultracentrifugatioh to isolate various organelles. The organelles were then viewed through a microscope. The drawing below was sketched based on a picture from a high-powered microscope. i. Name the type of high-powered microscope used to take the photograph. ii Give one evidence for your answer. When the isolation technique for organelles was repeated using the same liver tissue for a second time, all of the above organelles had burst. Infer one possible mistake that had been made during the isolation stage.Recall from the video the steps of how to make a wet mount of cheek epithelial cells. Place the steps of how to prepare a wet mount in the correct order from left • View Available Hint(s) Reset Help Add a drop of physiological saline to the center of the slide. Scrape the inside of the cheek with a toothpick. Stir the end of the toothpick in the physiological saline. Mix a drop of stain into the specimen preparation with a toothpick. Place a coverslip on top of the specimen.Match the cell component with its role in the cell. I have attached 2 images. please look at them