3. Acetobacter aceti is grown under effective anaerobic fermentation to obtain a starter culture for the production of vinegar. The cells synthesis acetic acid as a by-product of energy metabolism. The system has the properties below: Yxs = 0.23 kg kg-1 Ks 0.15 kg m³ |max = 0.35 h- ms 0.135 kg kg‍¹ hr¹ Fed-batch fermentation is operated in stirred tank fermenter under quasi steady state conditions with a feeding flow rate of 4 m³ h¹ and feeding substrate concentration of 80 kg m³. After 6 h, the liquid medium is 40 m³. a. Calculate the initial culture volume. b. Calculate the substrate concentration at quasi steady state. c. Calculate the cell concentration at quasi steady state. d. Calculate the cell mass produced after 6 h of feeding operation.

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3. Acetobacter aceti is grown under effective anaerobic fermentation to obtain a starter
culture for the production of vinegar. The cells synthesis acetic acid as a by-product of
energy metabolism. The system has the properties below:
Yxs = 0.23 kg kg-1
Ks 0.15 kg m³
|max = 0.35 h-
ms 0.135 kg kg‍¹ hr¹
Fed-batch fermentation is operated in stirred tank fermenter under quasi steady state
conditions with a feeding flow rate of 4 m³ h¹ and feeding substrate concentration of
80 kg m³. After 6 h, the liquid medium is 40 m³.
a. Calculate the initial culture volume.
b. Calculate the substrate concentration at quasi steady state.
c. Calculate the cell concentration at quasi steady state.
d. Calculate the cell mass produced after 6 h of feeding operation.
Transcribed Image Text:3. Acetobacter aceti is grown under effective anaerobic fermentation to obtain a starter culture for the production of vinegar. The cells synthesis acetic acid as a by-product of energy metabolism. The system has the properties below: Yxs = 0.23 kg kg-1 Ks 0.15 kg m³ |max = 0.35 h- ms 0.135 kg kg‍¹ hr¹ Fed-batch fermentation is operated in stirred tank fermenter under quasi steady state conditions with a feeding flow rate of 4 m³ h¹ and feeding substrate concentration of 80 kg m³. After 6 h, the liquid medium is 40 m³. a. Calculate the initial culture volume. b. Calculate the substrate concentration at quasi steady state. c. Calculate the cell concentration at quasi steady state. d. Calculate the cell mass produced after 6 h of feeding operation.
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