2. You take milk out of the refrigerator and pour it on your cereal. Clumps of milk pour out of thecarton onto your cereal. Which two biochemical concepts can explain why the milk formedclumps?A) Unfolding and oligomeric gel formation from mild heat (from someone leaving the milk out).B) Bacterial homeostasis and acid-base equilibrium using the Henderson-Hasselbalch equation.C) Bacterial acidification and protein aggregation at the isoelectric point.D) Hydrogen bonding between protein molecules and exponential bacterial growth.E) Sulfur production from thiotrophic bacteria and covalent disulfide bonding.3. Phosphoric acid is tribasic, with pka's of 2.14, 6.86, and 12.4. The ionic form that predominates atpH 6.86 is:А) НЗРОД.В) Н2РО4C) HPO,²-D) PO,3-E) Two forms are present in equal amounts

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Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter2: Life, Chemistry, And Water

Section: Chapter Questions

Problem 11TYK

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Consider a crude extract with a mixture of the 5 proteins listed below. Protein A 4.5 pl 160 molecular weight, kDa Protein B 12.5 pl and 65 molecular weight Protein C 5.0 pl, 15 molecular weight Protein D 6.8 pl, 150 molecular weight Protein E 9.5 pl, 45 molecular weight You load this protein mixture onto an anion exchange column at pH 11. Next, you apply a "washing" step by passing through buffer at pH 11. Finally, for your elution step, you apply a pH gradient starting from pH 11 to pH 2.0 (A gradient buffer system allows you to gradually and continuously change the pH of your mobile phase starting from pH 11 up to pH 2). You load this same protein mixture onto a Size Exclusion column. Please indicate the order in which these proteins will elute for both. Group of answer choices Yes or No, please explain your answer. If your protein of interest is protein A, would using anion exchange column be completely successful at separating it from all the other proteins? Group of answer…
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3. Phosphoric acid is tribasic, with pK,'s of 2.14, 6.86, and 12.4. The ionic form that predominates pH 6.86 is: А) НЗРО. B) H2PO4 C) HPO42- D) PO43- E) Two forms are present in equal amounts