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- Why are buffers important in biochemical experiments? Please explainw ellAn import relationship that allows us to determine the pKa of a weak acid using a pH titration curve is: pH = pKa (at the half-equivalence point) Explain which chemical species are present at this point in a WA/SB titration that makes this relationship true.1. Acids and Bases a. How reliable are the indicators (red cabbage and phenolphthalein) in the testing of these household substances? b. Suggest how these tests be converted to specific (numeric) pH values.
- whenanalyzing urine from a patient with kidney disease which organic molecules might be detected which biochemical test will be the most appropriate for detectionof those molecues?Quantitative Estimation of Amino Acids by Ninhydrin http://vlab.amrita.edu/?sub=3&brch=63&sim=156&cnt=2 can u help me with question 2 of the assignment questions Based on the experimental data provided, estimate the amount of amino acid in the given unknown solution by Ninhydrin method. SI No. Volume of standard amino acid solution (ml) Amount of amino acid (µg) OD at 570nm 1 Blank 0 2 0.2 0.12 3 0.4 0.25 4 0.6 0.45 5 0.8 0.55 6 1.0 100 0.68 7 Unknown (0.5ml) 0.41Calculate the pH of a mixture of 0.25 M acetic acid and 0.20 M sodium acetate. The pKa of acetic acid is 4.76.
- A mixture containing glutamic acid, arginine, phenylalanine and valine was subjected to anion exchange chromatography at pH 7.0. Which of the amino acids interacted with the resin (anion exchanger) and last to be eluted with a buffer of decreasing pH? A. Glu B. Val C. Phe D. ArgGiven ß- Cyclodextrin Briefly explain its expected reaction (based on their structural formula) to the different qualitative tests for carbohydrates which are: (a) Molisch’s test (b)Fehling’s test (c) Bial’s testCalculate the theoretical pH of a Tris buffer (pH 8.0) at 0 ºC. Assume that room temperature is 22 ºC
- Calculate the concentration of Y4-ion in 0.0100 M EDTA solution at pH 6. for pH 6, α4 = 2,2 x 10-5Explain how acetaminopen gives such spectroscopic and chromatographic graph by interpreting the peaks that appeared in the graphs. Fig1. Mass Spectrometric Chromatogram of Acetaminophen (the two graphs) Fig2. IR Spectrograph of AcetaminophenWhich organic molecules may be found in urine from a patient with kidney illness, and which biochemical test would be the most suited for detecting those molecues?