purpose(s) of DNA extraction
Q: Next generation sequencing using Illumina platforms identifies individual bases in a DNA fragment…
A: The following steps are involved in next generation sequencing: 1) First the DNA sequencing…
Q: 5) PCR primers have a melting temperature (Tm) at which they become dissociated from the template…
A: A primer is a short strand of nucleotide molecules that serves as an initial point for the synthesis…
Q: 1. (a) Restriction sites are usually ______. Recombinant DNA Technology Restriction…
A: Given, 1. (a) Restriction sites are usually ______. Recombinant DNA Technology…
Q: What causes the DNA to migrate from the wells toward the positive electrode? a) DNA is positively…
A: ANSWER;- b) DNA is negatively charged, and so was attracted to the positive electrode Explain;- DNA…
Q: 1. a) A technologist has a 20µl sample of DNA and adds 5µl of loading dye before adding the total…
A: Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: 2. a. Research DNA quantitation by UV absorption. Explain what A230, A260 and A280 values represent.…
A: Every biomolecule have an unique absorbance curve of their own. This simply means that, the…
Q: Which of the following actions in the DNA extraction of strawberry separates the DNA from the rest…
A: You have asked mutiple questions. I will answer the 1st question, as allowed by guidelines. Asked…
Q: 1. Provide one reason to support the creation of a national DNA database and one reason to refute…
A: DNA or deoxyribonucleic acid is the genetic material in all living organisms. Within the cells, DNA…
Q: Which of the following alternative steps cannot be employed in the DNA extraction because it would…
A: You have asked multiple questions. I will answer 1st question, as allowed by guidelines. Asked :…
Q: #1 under Identify the Structures is what.... A. original strands of DNA B. backbone of DNA C.…
A: The deoxyribonucleic acid (DNA) is the double-stranded molecule which is the genetic material in…
Q: 1. What is the function of the electrophoresis buffer? 2. Why did you mix your samples with loading…
A: Gel electrophoresis: it is a technique used to sepals different fragments of different lengths of a…
Q: 18. An instructor had her students perform this laboratory beginning with setting up their own…
A: Agarose gel electrophoresis is the technique for the separation of DNA fragments on the basis of…
Q: 4. The image below is based on the Meselson Stahl experiment where bacteria are grown in 15N and…
A: The Meselson-Stahl experiment showed that DNA replication is a semiconservative process. In this…
Q: If you will use apple or strawberries instead of banana do you think the result would be the same in…
A: All living things pass hereditary information from one generation to another using DNA as the basic…
Q: TRUE OR FALSE: a) Okazaki fragments are short DNA pieces that explain how the DNA polymerase can…
A: DNA replication process is semi conservative. The semi conservative replication of DNA refers to the…
Q: What should you do when loading the DNA sample into the gel well? A. Ensuring that the DNA samples…
A: Gel electrophoresis is a technique used in the biotechnology lab to separate the DNA fragments…
Q: Which of the folllowing is the term used for ultra compact form of DNA coiling? a. supercoiling…
A: Compacting DNA is important for packaging it within the cells. As the length of a DNA molecule can…
Q: 5. Below is an image of DNA sequenced using the Sanger sequencing method, where different…
A: Gel electrophoresis is a molecular technique that aims to separate the DNA, RNA, and protein…
Q: . Illustrate the basic steps in DNA extraction
A: The genetic material that is densely packed inside the nucleus and chromosomes is known as DNA. The…
Q: When working with the gel electrophoresis chamber, what must we keep in mind? A. Keeping the gel…
A: Gel electrophoresis technique of separation of molecules on the basis of their size and charge under…
Q: What does the salt in the soapy salt solution do in the DNA extraction? a. It dissolves the DNA. b.…
A: DNA molecule is a negatively charged molecule which is made up of nucleotides. Each nucleotide is…
Q: Which is the odd one out ? For the rest, explain the concept/process/technique they are involved…
A: DNA Polymerase is the main enzyme of replication. It adds deoxyribonucleotides according to the…
Q: When we discuss PCR and other similar techniques, the term Tm is often used. This refers to a.The…
A: PCR ( Polymerase Chain Reaction) is a technique in which numerous copies of genetic material (…
Q: 6) In automated DNA sequencing a) Radio labelled DNTPS are used b) Radio labelled ddNTPs are used c)…
A: Automated DNA sequencing is used to generate the nucleotide sequence of a given DNA strand. The…
Q: 4. DNA dissolves in water, but not in ethanol. Explain what happened when the ice cold ethanol came…
A: DNA is the genetic material and it is found in most organisms. DNA extraction is one of the first…
Q: fa restriction endonuclease recognizes and cleaves a linear piece of DNA and Circular DNA at 8…
A: Restriction endonuclease These are also known as restriction enzymes. These enzymes have been…
Q: 1. what are the three main steps of a solid phase DNA extraction? Answer is Lysis, but does not…
A: “Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: What is the purpose of cell dissolution? 2. What is the purpose of DNA separation? 3. What is the…
A: Cell lysis : The method in which the cell membrane is broken down to release the inter-cellular…
Q: What happens if you forget to add Sybrsafe to a gel that will be used for DNA gel electrophoresis…
A: Gel electrophoresis is a gel technique that separates DNA and proteins based on their mass, by means…
Q: 1. The polymerase chain reaction (PCR) is used by scientists to amplify DNA, particularly when the…
A: We are answering 1st question only. For the rest of the questions please repost. The polymerase…
Q: )Which of the following is not an application of PCR? a) Determine if two people are related. b)…
A: PCR(polymerase chain reaction) is a quantitative technique responsible for the amplification of…
Q: n Cohen-Boyer’s recombinant DNA procedure, ___i___ must be used for both the bacterial DNA and the…
A: The Cohen-Boyer experiment was to construct recombinant plasmids carrying the rRNA gene from the…
Q: Answer the following questions about Extraction of DNA A. (3) types of DNA B. Reagents needed to…
A: Nucleic acids are macromolecules or biopolymers made up of monomers called nucleotides.…
Q: What is a DNA Ladder? a solution added to a DNA sample to give it color for an electron microscope.…
A: DNA: The hereditary substance in humans and almost all other animals is DNA, or deoxyribonucleic…
Q: 1. Upon the banana DNA extraction, what do you see in the top portion of the liquid when you look at…
A: DNA (Deoxyribonucleic Acid) is the genetic material of all living organisms whether plant, animal,…
Q: 1. What is the basis of separation of different DNA fragments by gel electro- phoresis? a. The…
A: Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: 4. A linear fragment of DNA is exposed to a digest containing the individual restriction enzymes…
A: For doing restriction mapping we need to arrange each fragments at it's proper position. The process…
Q: 1 DNA evidence analysis is an imperfect science. Provide two reasons why this may be the case.
A: DNA is the hereditary material present in organisms. It is important for our growth, reproduction,…
Q: Rosalind Franklin (Early 1950s) A. What did she discover about the probable shape of DNA? B. What…
A: The experimental science that determines crystal atomic and molecular structure. This crystalline…
Q: 1. a. Draw the primers for this longer strand (consider optimal length):…
A: Introduction PCR is the revolutionized technique invented by Karry Mullis in 1983, for which he got…
Q: The extraction of DNA is a common procedure in biotechnology research laboratories. Research the…
A: Common DNA extraction methods Different extraction methods result in different yields and purity of…
Q: 1) The Qiagen DNeasy DNA extraction kit is used to extract DNA from a liver cell line for subsequent…
A: DNA extraction aids in the study of the genetic origins of different illnesses. For the development…
Q: Which of the following is the correct order in extracting the DNA? I. Washing of DNA II. Tissue…
A: DNA act as genetic material in most of organism . It is two stranded , ladder like structure . DNA…
Q: These highly polymorphic molecular markers are useful in DNA fingerprinting: (a) plasmid vectors (b)…
A: DNA typing is a laboratory procedure for detecting normal variations in a DNA sample…
Q: What is the purpose of restriction enzyme? 2. What is the purpose of the probe in DNA finger…
A: Since you have posted a question with multi- sub parts , we will solve first three sub-parts for…
Q: Some recombinant DNA techniques depend on the specific hybridization (or annealing) between two…
A: Recombinant DNA technology comprises altering genetic material outside an organism to obtain…
Q: 1.) What are the different temperatures used in PCR (polymerase chain reaction)? What happens at…
A: Polymerase Chain Reaction or PCR is a technique that used for making copies or amplify small…
1. What is/are the purpose(s) of DNA extraction?
2. What is the function of each reagent used in the extraction of DNA from a banana?
a. Saltwater
b. Liquid dishwashing soap
c. Cold isopropyl alcohol
Step by step
Solved in 2 steps
- n gel electrophoresis of DNA, the different bands in the final gel form because the DNA molecules _______. a. are from different organisms b. have different lengths c. have different nucleotide compositions d. have different genesMatch the method with the appropriate enzyme. _____ PCR a. Taq polymerase _____ cutting DNA b. DNA ligase _____ cDNA synthesis c. reverse transcriptase _____ DNA sequencing d. restriction enzyme _____ pasting DNA e. DNA polymerase (not Taq)After a polymerase chain reaction (PCR), agarose gel electrophoresis is often used to: a. amplify the DNA. b. convert cDNA into genomic DNA. c. convert cDNA into messenger RNA. d. verify that the desired DNA sequence has been amplified. e. synthesize primer DNA molecules.
- 1. (a) Restriction sites are usually ______. Recombinant DNA Technology Restriction enzymes Ligase Palindromic sequences (b) Involves joining a donor DNA fragment of interest to a vector Recombinant DNA Technology Restriction enzymes Ligase Palindromic sequencesWhich of the following is FALSE about current Sanger dideoxy DNA sequencing procedures? a. Chain termination occurs during synthesis of a new DNA strand. b. Many steps can be automated. c. No DNA is synthesized in the procedure. d. Fluorescent molecules can be used to detect the DNA.Explain. 1. What is the Difference between DNA Purification and RNA Purification? 2. why contaminants must be avoided in DNA samples? 3. Give 5 examples of sources of error in using a spectrophotometer.
- 1. What is the function of the DNA polymerase enzyme in the PCR? 2. What natural process is PCR based on?1. a) A technologist has a 20µl sample of DNA and adds 5µl of loading dye before adding the total volume into an agarose gel. What was the original concentration of the loading dye? a. 4x b. 5x c. 6x d. 7x e. None of the above 1. b)A technologist adds 6uL of 5x loading dye to a DNA sample before adding the total volume into the agarose gel. How much DNA sample was combined with the loading dye? 12uL 16uL 20uL 24uL 28uL1. Next generation sequencing using Illumina platforms identifies individual bases in a DNA fragment through a.chain termination b.sequencing by ligation c.sequencing by synthesis d.pyrosequencing
- When working with the gel electrophoresis chamber, what must we keep in mind? A. Keeping the gel flat on the table without any buffer solution B. Not changing your pipette in between DNA samples C. Not shaking the chamber after the DNA has been loaded D. Loading two DNA samples per a single well1. Pharmaceutical industry a. The importance of recombinant DNA technology in pharmaceutical industry b. Potential products produced and processes involved in pharmaceutical industry (at least 5 examples in each field). c. The downside/ disadvantages of recombinant DNA technology in pharmaceutical industry2. What does the salt in the soapy salt solution do in the DNA extraction? a. It dissolves the DNA. b. It displaced a lot of proteins to free the DNA. c. It disrupts the phospholipid bilayer. d. It precipitates the DNA.
