6. What is the function of the iodine solution in the Gram stain? If it wereomitted, how would staining results be affected?7. What is the purpose of the alcohol solution in the Gram stain?8. What counterstain is used in Gram staining? Why is it necessary? Couldcolors other than Safranin red be used?

Gram stain is a technique used for staining bacteria in tissues.

Answered: 6. What is the function of the iodine…

Phlebotomy Essentials

6th Edition

ISBN:9781451194524

Author:Ruth McCall, Cathee M. Tankersley MT(ASCP)

Publisher:Ruth McCall, Cathee M. Tankersley MT(ASCP)

Chapter1: Phlebotomy: Past And Present And The Healthcare Setting

Section: Chapter Questions

Problem 1SRQ

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Similar questions

1.)What is the purpose of a counterstain? 2. What does a mordant do in the Gram stain procedure? Which reagent in the Gram stain is the mordant? 3. True or False? The oil objective should make contact with the oil on the slide. 4. Why is it necessary to let bacterial smears completely air dry before heat fixing? 5.Why should controls be included wherever possible for any staining technique? 6. Why is it necessary to heat the slides while staining for endospores?
3. Draw the specimen observed under endospore staining 4. Draw the specimen observed under positive simple staining
2. What does a mordant do in the Gram stain procedure? Which reagent in the Gram stain is the mordant?
1. You accidentally used safranin as the primary stain and malachite green as the counter stain during a spore staining procedure. Explain how your microscopic observation would differ from those observed when slides of spore-containing bacteria were prepared correctly. 2.One of your classmates performed a gram stain on Pseudomonas aeruginosa and found variable gram staining, with only some of the cells gram positive. What should your classmate have found? What errors could have contributed to the variable result?
-What are the steps of gram staining -Which of the four steps is the most important and why?- What would happen to the cells if you use too much alcohol?- What would happen if you would not use enough alcohol?- What would happen if you left out the application of safranin?
1. What is the purpose for using stains? What microbial characteristics can one ascertain from a simple stain? 2. Why is it necessary to make a heat-fix smear and what are the disadvantages of heat fixing? 3. what is the best age for your culture when performing a gram stain? Why? 4. Why is Gean staining considered a differential staining process? 5. What are some reasons a Gram positive cell might appear Gram negative?
4. What is the theory about the mechanism of the Gram-stain reaction? 5. What part of the bacterial cell is most involved with Gram staining, and why? Please provide a reference
2.How do gram positive and gram negative bacteria differ in cellular structure, and how does this contribute to their differential staining properties? 3.How does the age of a culture affect the gram stain reaction? What is an optimum culture age for a valid gram reaction? 4.Which step in the gram stain procedure is most prone to error?If done correctly how might that step affect the end result? 5.what is the function of mordant, and which reagent serves this purpose in the gram stain procedure? 6.List the reagents of the gram star technique in order and their general role in the staining process. 7.In what type of cell, gram -positive or gram-negative , would you find lipopolysaccharide in its cell wall?
‏list 4 advantages and 4 limitations of the Gram staining process
1.why is gram stain considered a differential stain? 2.How do gram positive and gram negative bacteria differ in cellular structure, and how does this contribute to their differential staining properties? 3.How does the age of a culture affect the gram stain reaction? What is an optimum culture age for a valid gram reaction? 4.Which step in the gram stain procedure is most prone to error?If done correctly how might that step affect the end result? 5.what is the function of mordant, and which reagent serves this purpose in the gram stain procedure? 6.List the reagents of the gram star technique in order and their general role in the staining process. 7.In what type of cell, gram -positive or gram-negative , would you find lipopolysaccharide in its cell wall?
1. why is it necessary to employ a spore stain rather than rely on diagnosis of sporing from examination of gram stain 2.What is the purpose of the bacterial capsule?
1) Outline or discuss what happens to a gram negative and gram positive cell at EACH Individual step during the staining procedure. 2) What is a gram variable and why can gram variability occur?

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