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7. The reduced form of nicotinamide adenine dinucleotide (NADH) is an important and highly fluorescent coenzyme. It has an absorption maximum of 340 nm and an emission maximum at 465 nm. Standard solutions of NADH gave the following fluorescence intensities:

7. The reduced form of nicotinamide adenine dinucleotide (NADH) is an important and highly fluorescent coenzyme. It has an absorption maximum of 340 nm and an emission maximum at 465 nm. Standard solutions of NADH gave the following fluorescence intensities:

Fundamentals Of Analytical Chemistry
Fundamentals Of Analytical Chemistry
9th Edition
ISBN:9781285640686
Author:Skoog
Publisher:Skoog
Chapter6: Random Errors In Chemical Analysis
Section: Chapter Questions
Problem 6.17QAP
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Mulecular Absorption Spectrometry MacBook Pro 6. Describe the advantage of a four-level laser system over a three-level type. 7. The reduced form of nicotinamide adenine dinucleotide (NADH) is an important and highly fluorescent coenzyme. It has an absorption maximum of 340 nm and an emission maximum at 465 nm. Standard solutions of NADH gave the following fluorescence intensities: Relative Intensity Conc. NADH, umol/L 0.100 2.24 0.200 4.52 0.300 6.63 9.01 0.400 0.500 10.94 0.600 13.71 0.700 15.49 0.800 17.91 A) Construct a spreadsheet and use it to draw a calibration curve for NADH. B) Find the least-squares slope and intercept for the plot in (A) C) An unknown exhibit a relative fluorescence intensity of 12.16. Use the spreadsheet to calculate the concentration of NADH. 8. The IR spectrum of CO shows a vibrational absorption band centered at 2170/cm. (a) what is the force constant for the CO bond? (b) At what wavenumber would the corresponding peak for "CO occur? 9. Calculate the relative number of protons in the higher and lower magnetic states where sample is placed in a 5T field at 25°C. 10. Calculate the energy (in kJ/mol) that electrons acquire as a result of being accelerated through a potential of 80 V. (b) How does this energy compare to that of a typical chemical bond?
Transcribed Image Text:Mulecular Absorption Spectrometry MacBook Pro 6. Describe the advantage of a four-level laser system over a three-level type. 7. The reduced form of nicotinamide adenine dinucleotide (NADH) is an important and highly fluorescent coenzyme. It has an absorption maximum of 340 nm and an emission maximum at 465 nm. Standard solutions of NADH gave the following fluorescence intensities: Relative Intensity Conc. NADH, umol/L 0.100 2.24 0.200 4.52 0.300 6.63 9.01 0.400 0.500 10.94 0.600 13.71 0.700 15.49 0.800 17.91 A) Construct a spreadsheet and use it to draw a calibration curve for NADH. B) Find the least-squares slope and intercept for the plot in (A) C) An unknown exhibit a relative fluorescence intensity of 12.16. Use the spreadsheet to calculate the concentration of NADH. 8. The IR spectrum of CO shows a vibrational absorption band centered at 2170/cm. (a) what is the force constant for the CO bond? (b) At what wavenumber would the corresponding peak for "CO occur? 9. Calculate the relative number of protons in the higher and lower magnetic states where sample is placed in a 5T field at 25°C. 10. Calculate the energy (in kJ/mol) that electrons acquire as a result of being accelerated through a potential of 80 V. (b) How does this energy compare to that of a typical chemical bond?
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