Answered: A 20 ppm solution of a DNA molecule…

A 20 ppm solution of a DNA molecule (unknown molecular weight) isolated from Escherichia coli was found to an absorbance of 0.80 in a 2-cm cell. Calculate the absorptivity of the molecule.

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter13: An Introduction To Ultraviolet-visible Molecular Absorption Spectrometry

Section: Chapter Questions

Problem 13.24QAP

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Applications of Molecular Spectroscopy

When light sources pass through the diffraction grating then light splits into different wavelengths. The light source produces two types of patterns that are shown as destructive and non-destructive. The light source is in the form of quanta that is quantitation.

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A 20 ppm solution of a DNA molecule (unknown molecular weight) isolated from Escherichia coli was found to an absorbance of 0.80 in a 2-cm cell. Calculate the absorptivity of the molecule.

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