Answered: alculate the molar extinction…

alculate the molar extinction coefficient of a biomolecule with a MW of 300 if a 5.0 mM solution in a 1.2 cm cuvette has an absorbance of 0.50 at 340 nm.

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter7: Components Of Optical Instruments

Section: Chapter Questions

Problem 7.20QAP

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Concept explainers

UV Visible Spectroscopy

Spectroscopy refers to the absorption or emission of light or other radiations by matter. When a substance or chemical absorbs light or radiation, they reach an excited state followed by de-excitation or emission of the light or radiation forming distinct spectra. UV-visible spectroscopy or ultraviolet-visible refers to the absorption and emission in the ultraviolet and visible regions of the electromagnetic spectrum.

Question

Calculate the molar extinction coefficient of a biomolecule with a MW of 300 if a 5.0 mM solution in a 1.2 cm cuvette has an absorbance of 0.50 at 340 nm.

Carbon-based molecules made by living things. The four categories of biomolecules—proteins, lipids, nucleic acids, and carbohydrates—compose living things and carry out their chemical processes.

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