a) A plasmid DNA in bacteria has a length of 14,000 bp and an Lk of 1300. Calculate the superhelical density o for this plasmid. Show your work for partial credit, round to one digit after the decimal point. b) You use a Type Il topoisomerase to change the linking number of this plasmid to 1310. How many turnovers must the topoisomerase perform? Is this resulting plasmid underwound or overwound?
Q: referred to feedback inhibition as “Goldilocks regulation”. Describe how feedback inhibition keeps…
A: Biochemical pathways and reactions are occurring constantly in cells. These pathways convert the…
Q: 10 of these are soluble proteins and 10 are integral membrane proteins. Group (cut & paste, circle…
A: Integral membrane proteins are those that will be found spanning a membrane. They will have regular…
Q: II. True or False a. An effort is usually made to fragment the polypeptide backbone first, before…
A: Proteins are the heteropolymers of twenty standard amino acids that differ from each other on the…
Q: please check if my answers are correct. Especially for prolin and Leucine
A: Amino acids are biomolecules that have an amino group and a carboxyl group attached to the same…
Q: Which of the following single-stranded DNA molecules are likely to pair with each other to form…
A: DNA are polymers of nucleotides. A nucleotide consists of a nitrogenous base(A, T, G, C) attached to…
Q: Draw the structure of a polymer found in our body with 1-4 linkage. Just draw the repeating units.
A: The backbone glycosidic linkage of three abundant biological storage polysaccharides: amylose,…
Q: in the series: SCD, TEF, UGH, ___, WKL what letters should fill the blank
A: The first letter of an element in the series is consecutive to the previous elements first letter…
Q: can water be used as a biological buffer? why?
A: A buffer is a solution that resists the change in the pH. Usually buffers are made up weak acids and…
Q: In this FRET data table, an antibiotic was added, but the type was not recorded. According to the…
A: FRET (Förster resonance energy transfer) is a technique in which energy transfer between two…
Q: 1. Consider the enzyme pyruvate carboxylase. a. What pathway(s) does this enzyme function in? b.…
A: “Since you have posted a question with multiple sub-parts, we will solve the first four sub-parts…
Q: oles of: • Proline • Hydroxyproline • Hydroxylysine
A: Amino acids are biomolecules in which an amino group and a carboxyl group are linked to the same…
Q: The octapeptide gly-cys-met-asn-lys-ala-tyr-gly was hydrolyzed consecutively by CNBr and then…
A: The octapeptide gly-cys-met-asn-lys-ala-tyr-gly was hydrolyzed consecutively by CNBr and then…
Q: In bacteria, one of the main functions of pilli is A. Buoyancy B. Conjugation (genetic exchange) C.…
A: Based on nutritional mode, bacteria can be classified as autotrophic bacteria (those that can…
Q: 1. What happens to the pyruvate made during glycolysis under anaerobic conditions? Is this a redox…
A: Glycolysis is the breakdown of one molecule of glucose (6C) into two molecules of pyruvate (2 x 3C)…
Q: ATP is deemed the Universal currency for Cell, why do you think now it is the Fact
A: Energy-rich compounds are chemicals with specific structural characteristics that cause an energy…
Q: Proteins in/on target cells that bind messenger molecules and cause a response in the cells are…
A: Proteins are made up of amino acids which are used as a monomer unit of proteins. 22 standard…
Q: 5. Bread contains mixture of polypeptides known as gluten. This polypeptide has two types which are…
A: The folding of a polypeptide chain into a three dimensional folded conformation can be divided into…
Q: How can one confirm/investigate the type of inhibition exerted by AZT on the HIV reverse…
A: HIV stands for human immunodeficiency virus which causes AIDS and interferes with the body's ability…
Q: Give the structure and the corresponding function of Inulin.
A:
Q: Show the Hydrolysis of a dipeptide containing the amino acids threonine and methionine.
A: Amino acids are joined together in a linear manner by peptide linkage, known as the primary…
Q: Energy is stored long-term in the bonds of _____ and used short-term to perform work from a(n)…
A: A molecule is defined in chemistry as a grouping of distinct atoms. Water molecules (H2O) and carbon…
Q: CASE STUDY 9.5, PART 2 Recall Myra. Her mother was contacted by the EMTS, and she mentioned that her…
A: Diabetes mellitus is a clinical condition characterized by hyperglycemia and high blood sugar…
Q: actate dehydrogenase is a tetramer of MW 134000 g/mole composed of subunits which are equal in size.…
A: Lactate dehydrogenase belongs to the family of oxidoreductases. It catalyzes the conversion of…
Q: Draw the Gluconeogenesis pathway (pyruvate to glucose) and identify the different types of organic…
A: Introduction: The term gluconeogenesis refers to the production of glucose or glycogen from…
Q: Gluconeogenesis Q3.4 - Considering that gluconeogenesis requires a net input of 4 ATP equivalents…
A: Gluconeogenesis is the process of formation of glucose from non-carbohydrate sources such as…
Q: 15.5 Identify the acid-base conjugate pairs in each of the following reactions: (a) CH₂COO + HCN (b)…
A: An acid is defined as the donator of protons and a base is defined as the acceptor of protons. Acid…
Q: 14. Vitamin P: functional groups, mechanism of action, biological role, sources, daily requirement,…
A: Previously, the term "vitamin P" was applied to a class of plant chemicals known as flavonoids.…
Q: What possible quiestions can be askeed under the following question: How can one inestigate the tyoe…
A: AZT is a anti-HIV drug. It is a nucleoside analogue. AZT is incorporated in the replicating DNA in…
Q: Which of the following statements about alpha-helix and beta-sheet structure are true? More than one…
A: A protein's function depends on its structure. There are four levels of protein structure: primary,…
Q: The side chain of which residue can be hydrogen bond donor: a. Thr b. Ala c. Ile d. Pro e.…
A: There are twenty standard amino acids that makeup all the proteins present in our body. These twenty…
Q: A generalized enzyme active site is shaped like a hemisphere with a radius of 45Å. The active site…
A: Given that the active site is made up of HAVARILKHAVARILKHAVARILK amino acids at pH 7.38. The net…
Q: What following statement is TRUE concerning motifs and domains? a) Both motifs and domains are…
A: The proteins are folded into their three dimensional structure in order to gain biological function.…
Q: Classify the 13 known vitamins into water-soluble or fat-soluble vitamins. Water-Soluble Vitamins…
A: A vitamin organic molecules essential for the proper functioning of the body. They are…
Q: 15. A substrate binds to enzyme active site. a) List the roles of serine, histidine and aspartic…
A: The enzyme has serine, histidine and aspartate in the active site. Serine proteases are protein…
Q: 3-1. For each of the given amino acids, write the equilib- rium reactions that would apply at a pH…
A: The proteins re composed of amino acids having an alpha amino, alpha carboxyl and a side chain. The…
Q: Very slight changes in the shape of an enzyme may allow the enzyme to continue to function but at a…
A: Enzymes are biocatalysts that catalyse biochemical reactions. Allosteric enzymes are enzymes whose…
Q: please draw anr label the 20 amino acids found in proteins as they would exist in an aqueous…
A: Amino acids are biomolecules that have an amino group and a carboxyl group linked to the same carbon…
Q: Single digest - Cla I What is the number of fragments? What are/is the size(s) of fragment(s)?
A: ClaI is a restriction endonuclease obtained from Caryophanon latum. Its recognition site is a…
Q: Consider the following peptide sequence: Met-Ser-Val-Thr-Ile-Lys-Ala-Cys-Leu-Ser-lle-Tyr-Phe-Ser…
A: Structure of a protein or a peptide is described in four levels: primary, secondary, tertiary and…
Q: Explain why based on the FRET data.
A: FRET (Fluorescence Resonance Energy Transfer) is a technique to tell whether proteins are…
Q: Peptide mass fingerprinting can be used to identify proteins. It is a method of: a.…
A: The proteins can be identified my various methods such as NMR, X-ray crystallography, protein…
Q: what 3 types of lipids exist? Give an example of each.
A: Introducion Lipid is a biomolecule consists of carbon, hydrogen, and oxygen. Lipids are water…
Q: The β-Sheet is formed mainly by a. hydrophobic interaction b. hydrogen bonding between the…
A: Alpha-helix and beta-sheets are types of secondary structures that are mainly found in protein…
Q: What are the monomers of DNA & RNA? What 3 parts do the monomers contain?
A: Introduction: Nucleic acids are large biomolecules that carry hereditary information for cellular…
Q: Assumed that you have isolated the protein cytochrome C from a cell lysate. Discuss the advantages…
A: Cytochrome C Cytochrome C is a protein that is loosely bound to the inner membrane of mitochondria…
Q: Draw the Citric acid cycle pathway and identify the different types of organic reaction mechanisms.…
A: Citric acid cycle involves 8 biochemical reactions that occur in a cyclic manner. The acetyl CoA…
Q: What are pantothenic acid and biotin? Explain how these molecules are important to cellular…
A: The cell perform multiple metabolic pathways in order to maintain the life processes. The major…
Q: Procedure 1. Place 2mL of milk in each of the 3 test tubes. 2. Heat one of the test tubes to boiling…
A: Test tube 1 contains once boiled (then cooled) milk , formaldehyde and methylene blue . Test tube 2…
Q: Why are the vmax and km for both graphs not the same since the LB is just the inverse of MM plot.…
A: Lineweaver Burk (LB) plot is the double reciprocal plot that is constructed by plotting the inverse…
Q: Regarding the physical condition (characteristics of the solution/environment) in which an enzyme…
A: Enzymes are large molecular weight proteins that catalyse biochemical reaction. An enzyme's…
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- You are cloning the genome of a new DNA virus into pUC18.You plate out your transformants on ampicillin plates containing X-gal and pick one blue colony and one white colony.When you check the size of the inserts in each plasmid (blueand white), you are surprised to fi nd that the plasmid fromthe blue colony contains a very small insert of approximately60 bp, while the plasmid from the white colony does notappear to contain any insert at all. Explain these results.A 2.0kb bacterial plasmid ‘BS1030’ is digested with the restriction endonuclease Sau3A; the plasmid map is depicted in the diagram below and the Sau3A (S) restriction sites are indicated. Which of the following DNA fragments do you expect to see on an agarose gel when you run Sau3A-digested plasmid ‘BS1030’ DNA? a. 250 bp, 450 bp, 550 bp, 1.1 kb, 1.5 kb and 2.0 kb b. 2.0kb c. 250 bp, 400 bp, 450 bp, 500 bp and 550 bp d. 100 bp, 200 bp, 250 bp, 400 bp, 500 bp and 550 bpIn a transformation experiment, donor DNA was obtained from aprototroph bacterial strain (a+b+c+), and the recipient was a tripleauxotroph (a-b-c-). What general conclusions can you draw aboutthe linkage relationships among the three genes from the followingtransformant classes that were recovered? a+ b- c- 180 a- b+ c- 150 a+ b+ c- 210 a- b- c+ 179 a+ b- c+ 2 a- b+ c+ 1 a+ b+ c+ 3
- Consider the following plasmid (size 8000 bp), with restriction sites at the positions indicated: (see image) a) This plasmid is digested with the enzymes listed below. Indicate how many fragments will begenerated in each case, and give the sizes of the fragments.PstIXhoICombination of PstI + XhoI + EcoRI (triple digest) b) Draw the banding pattern you would expect to observe if each of these digestions is loaded into a separate well of an agarose gel, and the fragments separated by electrophoresis. In the first well you load a DNA marker (M) containing fragments with sizes of 1000 bp, 2000 bp, 4000 bp and 8000 bp. c) This gel is transferred to a membrane in a Southern blot experiment, and hybridised to a radioactively labelled 200 bp probe, which anneals to the plasmid DNA at the position indicated on the diagram above. Draw the autoradiographic profile you would expect to observe for the membrane.This plasmid was digested using different restriction enzymes whose sites have been mapped. The plasmid is 7896 base pairs long. This is a long question so u can count this as two or even three but please answer the question? Determine the size (base pairs) and number of fragments that would be produced if the plasmid was digested with the following enzymes: a) EcoRI b) BamHI c) HindIII d) EcoRI and HindIII e)EcoRI, HindIII, and BamHI *Hint- this is actually an EASY question, since the restriction map is already drawn for you!which parts would a plasmid vector with 2500 bp have in E. coli
- U have the plasmid pUC18/19, which is a circular plasmid that consists of 2686 bp. What would the number of and length of the fragments be if you cut the plasmid with the following restriction enzymes or combination of enzymes? Give a schematic representation of the digestions. PscI & GsuI ______________________________________________________________ ScaI, PdmI & BsaXI ______________________________________________________________ ScaI, SspI & EheI ______________________________________________________________A plasmid vector pBS281 is cleaved by the enzymeBamHI (5′ G^GATCC 3′), which recognizes only onesite in the DNA molecule. Human DNA is digestedwith the enzyme MboI (5′ ^GATC 3′), which recognizes many sites in human DNA. These two digestedDNAs are now ligated together. Consider only thosemolecules in which the pBS281 DNA has been joinedwith a fragment of human DNA. Answer the following questions concerning the junction between thetwo different kinds of DNA. a. What proportion of the junctions between pBS281and all possible human DNA fragments can becleaved with MboI?b. What proportion of the junctions between pBS281and all possible human DNA fragments can becleaved with BamHI?c. What proportion of the junctions between pBS281and all possible human DNA fragments can becleaved with XorII (5′ C^GATCG 3′)?d. What proportion of the junctions between pBS281and all possible human DNA fragments can becleaved with BstYI (5′ R^GATCY 3′)? (R and Ystand for purine and pyrimidine, respectively.)e.…What is the velocity of movement (in micrometers per second) of DNA polymerase III holoenzyme relative to the template?
- If you use the pUC18 vector to clone in the MCS region, predict the following: a) Do bacteria that are blue in color have a cloned insert? b)Do bacteria that are white in color have a cloned insert? c) If you were to grow these cells on Chloramphenicol (an antibiotic), would the bacteria with the pUC plasmid grow? Why or Why not?A researcher has a Trp− auxotrophic strain of E. coliwith a mutation in a single gene. To identify thatmutant gene, she uses a genomic library made from a wild-type version of that same strain to find plasmids that rescue the mutant phenotype. The result issurprising. She recovers 10 plasmids that provide aTrp+ phenotype, but six of the plasmids contain geneX, while the other four contain gene Y. Our scientisthas encountered a phenomenon called multicopysuppression, related to the fact that plasmids are usually present in several copies per bacterium. Becausethe genes in the plasmids are present in more thantheir usual single copy in the bacterial chromosome,more than the usual amount of Protein X or Protein Yis being produced from the plasmids. Sometimes,overexpression of one protein can rescue the mutantphenotype caused by loss of a different protein.Suggest at least two ways that our scientist could determine which of the two genes, gene X or gene Y, actually corresponds to the…Consider the ends of the DNA fragments shown below. They have been produced by digestion of a single sequence of DNA using a number of restriction endonucleases. 1. 5'A 3' 3'TTCGA5' 2. 5'G 3' 3'CAGCT5' 3. 5'AATTC3' 3' G5 4. 5'TCGAC3' 3' G5' 5. 5'GGG 3' 3'CCC 5' Which of these ends are capable of annealing and being joined by DNA ligase?
